FAK/src-family Dependent Activation of the Ste20-like Kinase SLK is Required for Microtubule-dependent Focal Adhesion Turnover and Cell Migration

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2008 Apr 3

PMID 18382658

Citations 25

Authors

Simona Wagner

Chris J Storbeck

Kristin Roovers

Ziad Y Chaar

Piotr Kolodziej

Marlene Mckay

Luc A Sabourin

Affiliations

Soon will be listed here.

Abstract

Cell migration involves a multitude of signals that converge on cytoskeletal reorganization, essential for development, immune responses and tissue repair. Using knockdown and dominant negative approaches, we show that the microtubule-associated Ste20-like kinase SLK is required for focal adhesion turnover and cell migration downstream of the FAK/c-src complex. Our results show that SLK co-localizes with paxillin, Rac1 and the microtubules at the leading edge of migrating cells and is activated by scratch wounding. SLK activation is dependent on FAK/c-src/MAPK signaling, whereas SLK recruitment to the leading edge is src-dependent but FAK independent. Our results show that SLK represents a novel focal adhesion disassembly signal.

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