Effect of Antimicrobial Peptides Divergicin M35 and Nisin A on Listeria Monocytogenes LSD530 Potassium Channels

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2008 Apr 2

PMID 18379845

Citations 5

Authors

Karim Naghmouchi

Djamel Drider

Riadh Hammami

Ismail Fliss

Affiliations

Soon will be listed here.

Abstract

The aim of this work was to study the effect of antimicrobial peptides: divergicin M35 and nisin A on Listeria monocytogenes LSD 530 potassium (K+) channels: ATP-sensitive (K ATP), calcium-activated (BK Ca), and depolarization-activated (Kv) types. Increase on K+ efflux and inhibition of cellular growth were observed after adding K+ channel activators pinacidil, NS1619, and cromakalim to divergicin M35. Increase in K+ efflux from log-phase cells was about 18 +/- 1.1, 11 +/- 0.63, and nmol mg(-1) of cell dry weight (CDW) for pinacidil and NS1619, respectively, over the efflux obtained with divergicin M35 alone. Increases in K+ efflux obtained by adding the same K+ channel activators to nisin A fit a completely different profile. Divergicin M35 activates K+ channels, particularly of the Kv and BK Ca types and to a lesser extent the K ATP type, causing K+ efflux and consequently cell death.

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