Hypocretin-1 Potentiates NMDA Receptor-mediated Somatodendritic Secretion from Locus Ceruleus Neurons

Overview

Journal J Neurosci

Specialty Neurology

Date 2008 Mar 21

PMID 18354023

Citations 12

Authors

Xiao-Wei Chen

Yu Mu

Hong-Ping Huang

Ning Guo

Bo Zhang

Shuang-Yi Fan

Jia-Xiang Xiong

Shi-Rong Wang

Wei Xiong

Wei Huang

Tao Liu

Liang-Hong Zheng

Claire Xi Zhang

Li-Huan Li

Zheng-Ping Yu

Zhi-An Hu

Zhuan Zhou

Affiliations

Soon will be listed here.

Abstract

Our previous observations showed that several stimuli, including high-K(+) solution, glutamate, and voltage pulses, induce somatic noradrenaline (NA) secretion from locus ceruleus (LC) neurons. Hypocretin (orexin), a hypothalamic peptide critical for normal wakefulness, has been shown to evoke NA release from the axon terminals of LC neurons. Here, we used amperometry to test the effect of hypocretin-1 (HCRT) on NMDA receptor-mediated somatodendritic release in LC neurons. Either HCRT or NMDA applied alone dose-dependently induced somatodendritic secretion. Bath application of HCRT notably potentiated NMDA receptor-mediated somatodendritic NA release. This potentiation was blocked by SB 334867, a selective HCRT receptor (Hcrtr 1) antagonist, or bisindolylmaleimide, a specific protein kinase C (PKC) inhibitor, indicating the involvement of Hcrtr 1 and PKC. Consistent with this, phorbol 12-myristate 13-acetate, a PKC activator, mimicked the HCRT-induced potentiation. Furthermore, HCRT enhanced NMDA-induced intracellular Ca(2+) elevation via activation of Hcrtr 1 and PKC, which may contribute to HCRT-potentiated somatodendritic secretion. These results suggest that HCRT modulates LC activity not only by regulating noradrenergic input to its targets, but also by affecting noradrenergic communication in the soma and dendrites.

Citing Articles

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