Semi-degradable Scaffold for Articular Cartilage Replacement

Overview

Journal Tissue Eng Part A

Specialties Anatomy & Histology
Biomedical Engineering
Biotechnology

Date 2008 Mar 13

PMID 18333818

Citations 9

Authors

Devon C Charlton

Margaret G E Peterson

Kara Spiller

Anthony Lowman

Peter A Torzilli

Suzanne A Maher

Affiliations

Soon will be listed here.

Abstract

Existing technologies have not met the challenge of designing a construct for the repair of focal cartilage defects such that it mimics the mechanical properties of and can integrate with native cartilage. Herein we describe a novel construct consisting of a non-degradable poly-vinyl alcohol (PVA) scaffold to provide long-term mechanical stability, interconnected pores to allow for the infiltration of chondrocytes, and poly-lactic glycolic acid (PLGA) microspheres for the incorporation of growth factors to enhance cellular migration. The objective of this study was to characterize the morphological features and mechanical properties of our porous PVA-PLGA construct as a function of PLGA content. Varying the PLGA content was found to have a significant effect on the morphological features of the construct. As PLGA content increased from 10% to 75%, samples exhibited a 6-fold increase in average percentage porosity, an increase in average microsphere diameter from 8 to 34 microm and an increase in average pore diameter from 29 to 111 microm. The effect of PLGA content on aggregate modulus and permeability was less profound. Our findings suggest that that morphology of the construct can be tailored to optimize cellular infiltration and the dynamic mechanical response. The experiments herein presented were conducted at the Hospital for Special Surgery.

Citing Articles

Recent Developments and Current Applications of Organic Nanomaterials in Cartilage Repair.

Wei Z, Zhang G, Cao Q, Zhao T, Bian Y, Zhu W Bioengineering (Basel). 2022; 9(8).

PMID: 36004915 PMC: 9405275. DOI: 10.3390/bioengineering9080390.

The Use of Nanomaterials in Tissue Engineering for Cartilage Regeneration; Current Approaches and Future Perspectives.

Eftekhari A, Dizaj S, Sharifi S, Salatin S, Rahbar Saadat Y, Zununi Vahed S Int J Mol Sci. 2020; 21(2).

PMID: 31947685 PMC: 7014227. DOI: 10.3390/ijms21020536.

A Systematic Review and Guide to Mechanical Testing for Articular Cartilage Tissue Engineering.

Patel J, Wise B, Bonnevie E, Mauck R Tissue Eng Part C Methods. 2019; 25(10):593-608.

PMID: 31288616 PMC: 6791482. DOI: 10.1089/ten.TEC.2019.0116.

Supporting Biomaterials for Articular Cartilage Repair.

Duarte Campos D, Drescher W, Rath B, Tingart M, Fischer H Cartilage. 2015; 3(3):205-21.

PMID: 26069634 PMC: 4297118. DOI: 10.1177/1947603512444722.

The influence of IL-10 and TNFα on chondrogenesis of human mesenchymal stromal cells in three-dimensional cultures.

Jagielski M, Wolf J, Marzahn U, Volker A, Lemke M, Meier C Int J Mol Sci. 2014; 15(9):15821-44.

PMID: 25207597 PMC: 4200793. DOI: 10.3390/ijms150915821.

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