Enhanced Hematopoietic Activity of a Human Granulocyte/macrophage Colony-stimulating Factor-interleukin 3 Fusion Protein

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1991 Jul 1

PMID 1829529

Citations 18

Authors

B M Curtis

D E Williams

H E Broxmeyer

J Dunn

T Farrah

E Jeffery

W Clevenger

P deRoos

U Martin

D Friend

Affiliations

Soon will be listed here.

Abstract

Granulocyte/macrophage colony-stimulating factor-interleukin 3 (GM-CSF-IL-3) fusion proteins were generated by construction of a plasmid in which the coding regions of human GM-CSF and IL-3 cDNAs were connected by a synthetic linker sequence followed by subsequent expression in yeast. Both GM-CSF-IL-3 and IL-3-GM-CSF fusion proteins were purified to homogeneity and shown to bind to cell-surface receptors through either their GM-CSF or IL-3 domains. The fusion proteins exhibited enhanced receptor affinity, proliferative activity, and hematopoietic colony-stimulating activity compared with either IL-3 and/or GM-CSF alone. This suggests that GM-CSF-IL-3 fusion proteins may hold future promise as therapeutic agents.

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