The Fluidity of Normal and Virus-transformed Cell Plasma Membrane

Overview

Journal Biochem J

Specialty Biochemistry

Date 1976 Mar 15

PMID 182120

Citations 2

Authors

K J Micklem

R M Abra

S Knutton

J M Graham

C A Pasternak

Affiliations

Soon will be listed here.

Abstract

1. The phospholipid composition and cholesterol/phospholipid ratio of plasma membrane is the same in normal as in transformed BHK (baby-hamster kidney) cells; no significant difference in length or degree of unsaturation of the contributing acyl chains is apparent. 2. The turnover of acetate-labelled phosphatidylcholine species in the plasma membrane of normal and transformed BHK cells is the same. 3. Intramembranous particles of normal and transformed 3T3-cell plasma membrane are randomly distributed, whether at 4degreesC or at 37degreesC, in sparse or in dense cultures. There is no correlation between distribution of particles and the movement of concanavalin A receptor sites. 4. It is concluded that transformation of fibroblastic cells by oncogenic viruses does not lead to major changes in the lipid fluidity of the plasma membrane. 5. Details of the phospholipid composition of nuclei, mitochondria and endoplasmic reticulum in normal and transformed BHK cells have been deposited as Supplementary Publication SUP 50061 (5 pages) at the British Library Lending Division, Boston, Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies may be obtained on the terms given in Biochem. J. (1976) 153, 5.

Citing Articles

Regulation of passive potassium transport of normal and transformed 3T3 mouse cell cultures by external calcium concentration and temperature.

Ernst M, Adam G J Membr Biol. 1981; 61(3):155-72.

PMID: 6268792 DOI: 10.1007/BF01870521.

Differences in the phospholipid, cholesterol, and fatty acyl composition of 3T3 and SV3T3 plasma membranes.

Perkins R, Scott R Lipids. 1978; 13(10):653-7.

PMID: 214660 DOI: 10.1007/BF02533741.

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