Intramembrane Particle Aggregation in Erythrocyte Ghosts. I. The Effects of Protein Removal

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1974 Dec 1

PMID 4215819

Citations 71

Authors

A Elgsaeter

D Branton

Affiliations

Soon will be listed here.

Abstract

We have used freeze-etching and SDS-polyacrylamide gel electrophoresis to study the conditions under which the intramembrane particles of the human erythrocyte ghost may be aggregated. The fibrous membrane protein, spectrin, can be almost entirely removed from erythrocyte ghosts with little or no change in the distribution of the particles. However, after spectrin depletion, particle aggregation in the plane of the membrane may be induced by conditions which cause little aggregation in freshly prepared ghosts. This suggests that the spectrin molecules form a molecular meshwork which limits the translational mobility of the erythrocyte membrane particles.

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References

MARCHESI S, STEERS E, Marchesi V, TILLACK T . Physical and chemical properties of a protein isolated from red cell membranes. Biochemistry. 1970; 9(1):50-7. DOI: 10.1021/bi00803a007. View

GERATZ J . Inhibitory effect of aromatic diamidines on trypsin and enterokinase. Experientia. 1969; 25(12):1254-5. DOI: 10.1007/BF01897480. View

NIEHAUS Jr W, Wold F . Cross-linking of erythrocyte membranes with dimethyl adipimidate. Biochim Biophys Acta. 1970; 196(2):170-5. DOI: 10.1016/0005-2736(70)90004-0. View

Pinto da Silva P, Branton D . Membrane splitting in freeze-ethching. Covalently bound ferritin as a membrane marker. J Cell Biol. 1970; 45(3):598-605. PMC: 2107921. DOI: 10.1083/jcb.45.3.598. View

Frye L, Edidin M . The rapid intermixing of cell surface antigens after formation of mouse-human heterokaryons. J Cell Sci. 1970; 7(2):319-35. DOI: 10.1242/jcs.7.2.319. View

PALEK J, CURBY W, LIONETTI F . Effects of calcium and adenosine triphosphate on volume of human red cell ghosts. Am J Physiol. 1971; 220(1):19-26. DOI: 10.1152/ajplegacy.1971.220.1.19. View

Pinto da Silva P, Douglas S, Branton D . Localization of A antigen sites on human erythrocyte ghosts. Nature. 1971; 232(5307):194-6. DOI: 10.1038/232194a0. View

Fairbanks G, Steck T, Wallach D . Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971; 10(13):2606-17. DOI: 10.1021/bi00789a030. View

Steck T, Fairbanks G, Wallach D . Disposition of the major proteins in the isolated erythrocyte membrane. Proteolytic dissection. Biochemistry. 1971; 10(13):2617-24. DOI: 10.1021/bi00789a031. View

10.

JACOB H, Ruby A, Overland E, MAZIA D . Abnormal membrane protein of red blood cells in hereditary spherocytosis. J Clin Invest. 1971; 50(9):1800-5. PMC: 292104. DOI: 10.1172/JCI106670. View

11.

Clarke M . Isolation and characterization of a water-soluble protein from bovine erythrocyte membranes. Biochem Biophys Res Commun. 1971; 45(4):1063-70. DOI: 10.1016/0006-291x(71)90445-1. View

12.

Moor H . Recent progress in the freeze-etching technique. Philos Trans R Soc Lond B Biol Sci. 1971; 261(837):121-31. DOI: 10.1098/rstb.1971.0042. View

13.

Singer S, Nicolson G . The fluid mosaic model of the structure of cell membranes. Science. 1972; 175(4023):720-31. DOI: 10.1126/science.175.4023.720. View

14.

Staehelin L, Chlapowski F, BONNEVILLE M . Lumenal plasma membrane of the urinary bladder. I. Three-dimensional reconstruction from freeze-etch images. J Cell Biol. 1972; 53(1):73-91. PMC: 2108692. DOI: 10.1083/jcb.53.1.73. View

15.

Brown P . Rhodopsin rotates in the visual receptor membrane. Nat New Biol. 1972; 236(63):35-8. DOI: 10.1038/newbio236035a0. View

16.

Cone R . Rotational diffusion of rhodopsin in the visual receptor membrane. Nat New Biol. 1972; 236(63):39-43. DOI: 10.1038/newbio236039a0. View

17.

TILLACK T, Scott R, Marchesi V . The structure of erythrocyte membranes studied by freeze-etching. II. Localization of receptors for phytohemagglutinin and influenza virus to the intramembranous particles. J Exp Med. 1972; 135(6):1209-27. PMC: 2139169. DOI: 10.1084/jem.135.6.1209. View

18.

STIRLING C . Radioautographic localization of sodium pump sites in rabbit intestine. J Cell Biol. 1972; 53(3):704-14. PMC: 2108766. DOI: 10.1083/jcb.53.3.704. View

19.

Friend D, Gilula N . Variations in tight and gap junctions in mammalian tissues. J Cell Biol. 1972; 53(3):758-76. PMC: 2108762. DOI: 10.1083/jcb.53.3.758. View

20.

Pinto da Silva P . Translational mobility of the membrane intercalated particles of human erythrocyte ghosts. pH-dependent, reversible aggregation. J Cell Biol. 1972; 53(3):777-87. PMC: 2108782. DOI: 10.1083/jcb.53.3.777. View