Single-shot Detection of Bacterial Endospores Via Coherent Raman Spectroscopy

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2008 Jan 11

PMID 18184801

Citations 19

Authors

Dmitry Pestov

Xi Wang

Gombojav O Ariunbold

Robert K Murawski

Vladimir A Sautenkov

Arthur Dogariu

Alexei V Sokolov

Marlan O Scully

Affiliations

Soon will be listed here.

Abstract

Recent advances in coherent Raman spectroscopy hold exciting promise for many potential applications. For example, a technique, mitigating the nonresonant four-wave-mixing noise while maximizing the Raman-resonant signal, has been developed and applied to the problem of real-time detection of bacterial endospores. After a brief review of the technique essentials, we show how extensions of our earlier experimental work [Pestov D, et al. (2007) Science 316:265-268] yield single-shot identification of a small sample of Bacillus subtilis endospores (approximately 10(4) spores). The results convey the utility of the technique and its potential for "on-the-fly" detection of biohazards, such as Bacillus anthracis. The application of optimized coherent anti-Stokes Raman scattering scheme to problems requiring chemical specificity and short signal acquisition times is demonstrated.

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