Stability of the T-DNA Flanking Regions in Transgenic Arabidopsis Thaliana Plants Under Influence of Abiotic Stress and Cultivation Practices

Overview

Journal Plant Cell Rep

Publisher Springer

Date 2007 Dec 19

PMID 18087701

Citations 3

Authors

Nina Papazova

Rim Ghedira

Sabine Van Glabeke

Aghleb Bartegi

Pieter Windels

Isabel Taverniers

Isabel Roldan-Ruiz

Erik Van Bockstaele

Anne Milcamps

Guy Van den Eede

Ann Depicker

Marc De Loose

Affiliations

Soon will be listed here.

Abstract

Genetic transformation is often associated with different rearrangements of the plant genome at the site of insertion. Therefore the question remains weather these T-DNA insertion sites are more prone to genotoxic stresses. Here, we studied the impact of propagation through generations, the influence of gene stacking and of photo oxidative stress caused by high light intensity on the stability of the transgene flanking regions in the model plant Arabidopsis thaliana. Conformational Sensitive Capillary Electrophoresis (CSCE), RFLP and sequencing were deployed in this analysis in order to study the proximal 100 bp and the long-range T-DNA flanking sequences. By screening seven transgenic lines no evidence for occurrence of mutation events were found, implying that the nucleotide sequence of the T-DNA flanking regions of the studied events is unlikely to be unstable.

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