The Effect of Nomega-nitro-L-arginine Methyl Ester and L-arginine on Lung Injury Induced by Abdominal Aortic Occlusion-reperfusion

Overview

Journal Surg Today

Specialty General Surgery

Date 2007 Dec 19

PMID 18085359

Authors

Huseyin Okutan

Ilker Kiris

Ali K Adiloglu

Cagri Savas

Nilgun Kapucuoglu

Irfan Altuntas

Onur Akturk

Affiliations

Soon will be listed here.

Abstract

Purpose: The aim of this study was to examine the effects of Nomega-nitro-L-arginine methyl ester (L-NAME) and L-arginine on lung injury after aortic ischemia-reperfusion (IR).

Methods: Twenty-four Wistar-Albino rats were randomized into four groups (n = 6) as follows: Control (sham laparotomy), Aortic IR (30 min ischemia and 120 min reperfusion), L-Arginine (intraperitoneal 100 mg kg(-1) live weight)+aortic IR, and L: -NAME (intraperitoneal 10 mg kg(-1) live weight)+aortic IR. In the lung specimens, the tissue levels of malondialdehyde (MDA), vascular endothelial growth factor (VEGF), and nitric oxide (NO) were measured and a histological examination was done.

Results: Aortic IR increased MDA, VEGF, and NO. L-Arginine further significantly increased MDA and NO, and decreased VEGF (P < 0.05 vs aortic IR). L-NAME significantly decreased MDA and NO (P < 0.05 vs L-arginine+aortic IR) and increased VEGF (P < 0.05 vs other groups). A histological examination showed the aortic IR to significantly increase (P < 0.05 vs control) while L-arginine also further increased (P > 0.05 vs aortic IR), whereas L-NAME caused a significant decrease in pulmonary leukocyte infiltration (P < 0.05 vs aortic IR).

Conclusions: Our results indicate that L-arginine aggravates the lung injury induced by aortic IR, while L-NAME attenuates it.

References

Eschwege P, Paradis V, Conti M, Holstege A, Richet F, Deteve J . In situ detection of lipid peroxidation by-products as markers of renal ischemia injuries in rat kidneys. J Urol. 1999; 162(2):553-7. View

Mura M, Santos C, Stewart D, Liu M . Vascular endothelial growth factor and related molecules in acute lung injury. J Appl Physiol (1985). 2004; 97(5):1605-17. DOI: 10.1152/japplphysiol.00202.2004. View

Gelman S . The pathophysiology of aortic cross-clamping and unclamping. Anesthesiology. 1995; 82(4):1026-60. DOI: 10.1097/00000542-199504000-00027. View

Gross S, Wolin M . Nitric oxide: pathophysiological mechanisms. Annu Rev Physiol. 1995; 57:737-69. DOI: 10.1146/annurev.ph.57.030195.003513. View

Tuder R, Flook B, Voelkel N . Increased gene expression for VEGF and the VEGF receptors KDR/Flk and Flt in lungs exposed to acute or to chronic hypoxia. Modulation of gene expression by nitric oxide. J Clin Invest. 1995; 95(4):1798-807. PMC: 295709. DOI: 10.1172/JCI117858. View

May G, Crook P, Moore P, Page C . The role of nitric oxide as an endogenous regulator of platelet and neutrophil activation within the pulmonary circulation of the rabbit. Br J Pharmacol. 1991; 102(3):759-63. PMC: 1917930. DOI: 10.1111/j.1476-5381.1991.tb12246.x. View

Xu C, Zarins C, Glagov S . Aneurysmal and occlusive atherosclerosis of the human abdominal aorta. J Vasc Surg. 2001; 33(1):91-6. DOI: 10.1067/mva.2001.109744. View

Knowles R, Moncada S . Nitric oxide synthases in mammals. Biochem J. 1994; 298 ( Pt 2):249-58. PMC: 1137932. DOI: 10.1042/bj2980249. View

Mura M, Han B, Andrade C, Seth R, Hwang D, Waddell T . The early responses of VEGF and its receptors during acute lung injury: implication of VEGF in alveolar epithelial cell survival. Crit Care. 2006; 10(5):R130. PMC: 1751039. DOI: 10.1186/cc5042. View

10.

Sookhai S, Wang J, McCourt M, Kirwan W, Bouchier-Hayes D, Redmond P . A novel therapeutic strategy for attenuating neutrophil-mediated lung injury in vivo. Ann Surg. 2002; 235(2):285-91. PMC: 1422426. DOI: 10.1097/00000658-200202000-00018. View

11.

Kacmaz A, Polat A, User Y, Tilki M, Ozkan S, Sener G . Octreotide improves reperfusion-induced oxidative injury in acute abdominal hypertension in rats. J Gastrointest Surg. 2004; 8(1):113-9. DOI: 10.1016/j.gassur.2003.09.026. View

12.

Hoshida S, Yamashita N, Igarashi J, Nishida M, Hori M, Kamada T . Nitric oxide synthase protects the heart against ischemia-reperfusion injury in rabbits. J Pharmacol Exp Ther. 1995; 274(1):413-8. View

13.

Meyer K, Cardoni A, Xiang Z, Cornwell R, Love R . Vascular endothelial growth factor in human lung transplantation. Chest. 2001; 119(1):137-43. DOI: 10.1378/chest.119.1.137. View

14.

Ma T, Ischiropoulos H, Brass C . Endotoxin-stimulated nitric oxide production increases injury and reduces rat liver chemiluminescence during reperfusion. Gastroenterology. 1995; 108(2):463-9. DOI: 10.1016/0016-5085(95)90075-6. View

15.

Draper H, Hadley M . Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol. 1990; 186:421-31. DOI: 10.1016/0076-6879(90)86135-i. View

16.

Okutan H, Savas C, Delibas N . The antioxidant effect of melatonin in lung injury after aortic occlusion-reperfusion. Interact Cardiovasc Thorac Surg. 2007; 3(3):519-22. DOI: 10.1016/j.icvts.2004.05.005. View

17.

Pryor W, Squadrito G . The chemistry of peroxynitrite: a product from the reaction of nitric oxide with superoxide. Am J Physiol. 1995; 268(5 Pt 1):L699-722. DOI: 10.1152/ajplung.1995.268.5.L699. View

18.

Dusting G . Nitric oxide in cardiovascular disorders. J Vasc Res. 1995; 32(3):143-61. DOI: 10.1159/000159089. View

19.

Liu P, Xu B, Hock C . Inhibition of nitric oxide synthesis by L-name exacerbates acute lung injury induced by hepatic ischemia-reperfusion. Shock. 2001; 16(3):211-7. DOI: 10.1097/00024382-200116030-00007. View

20.

Somuncu S, Cakmak M, Erdogan S, Caglayan O, Caglayan F, Akman H . Protective effects of trapidil in lung after abdominal aorta induced ischemia-reperfusion injury: an experimental study. Pediatr Surg Int. 2005; 21(12):983-8. DOI: 10.1007/s00383-005-1542-2. View