Fast Enzyme Dynamics at the Active Site of Formate Dehydrogenase

Overview

Journal J Am Chem Soc

Publisher American Chemical Society

Specialty Chemistry

Date 2007 Dec 11

PMID 18067303

Citations 30

Authors

Jigar N Bandaria

Samrat Dutta

Sarah E Hill

Amnon Kohen

Christopher M Cheatum

Affiliations

Soon will be listed here.

Abstract

The role of femtosecond-picosecond structural dynamics of proteins in enzyme-catalyzed reactions is a hotly debated topic. We report infrared photon echo measurement of the formate dehydrogenase-NAD+-azide ternary complex. In contrast to earlier studies of protein dynamics, the data show complete spectral diffusion on the femtosecond-picosecond time scale with no static heterogeneity. This result indicates that this transition-state analogue complex completely samples the distribution of structures that determine the distribution of azide vibrational frequencies within a few picoseconds and that there are no slower motions that perturb the H-bond network at the active site.

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