» Articles » PMID: 18057129

Comparison of the BD GeneOhm Methicillin-resistant Staphylococcus Aureus (MRSA) PCR Assay to Culture by Use of BBL CHROMagar MRSA for Detection of MRSA in Nasal Surveillance Cultures from an At-risk Community Population

Overview
Specialty Microbiology
Date 2007 Dec 7
PMID 18057129
Citations 32
Authors
Affiliations
Soon will be listed here.
Abstract

We compared the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR assay to culture with BBL CHROMagar MRSA for nasal surveillance among 602 arrestees from the Baltimore City Jail. The sensitivity and specificity were 88.5% and 91.0%, respectively, and after secondary analysis using enrichment broth, they were 89.0% and 91.7%, respectively. Twenty-three of 42 false-positive PCR lysates contained methicillin-susceptible S. aureus.

Citing Articles

High frequency of occupied attB regions in Norwegian Staphylococcus aureus isolates supports a two-step MRSA screening algorithm.

Tunsjo H, Kalyanasundaram S, Worren M, Leegaard T, Moen A Eur J Clin Microbiol Infect Dis. 2016; 36(1):65-74.

PMID: 27638009 DOI: 10.1007/s10096-016-2771-0.


Performance of BD Max StaphSR for Screening of Methicillin-Resistant Staphylococcus aureus Isolates among a Contemporary and Diverse Collection from 146 Institutions Located in Nine U.S. Census Regions: Prevalence of mecA Dropout Mutants.

Mendes R, Watters A, Rhomberg P, Farrell D, Jones R J Clin Microbiol. 2015; 54(1):204-7.

PMID: 26537444 PMC: 4702731. DOI: 10.1128/JCM.02047-15.


Polymerase chain reaction-based active surveillance of MRSA in emergency department patients.

Seki M, Takahashi H, Yamamoto N, Hamaguchi S, Ojima M, Hirose T Infect Drug Resist. 2015; 8:113-8.

PMID: 25999747 PMC: 4437520. DOI: 10.2147/IDR.S80123.


Prevalence and risk factors for methicillin-resistant Staphylococcus aureus in an HIV-positive cohort.

Farley J, Hayat M, Sacamano P, Ross T, Carroll K Am J Infect Control. 2015; 43(4):329-35.

PMID: 25687358 PMC: 4386874. DOI: 10.1016/j.ajic.2014.12.024.


Laboratory evaluation of the BD MAX MRSA assay.

Widen R, Healer V, Silbert S J Clin Microbiol. 2014; 52(7):2686-8.

PMID: 24829235 PMC: 4097742. DOI: 10.1128/JCM.00237-14.


References
1.
de San N, Denis O, Gasasira M, De Mendonca R, Nonhoff C, Struelens M . Controlled evaluation of the IDI-MRSA assay for detection of colonization by methicillin-resistant Staphylococcus aureus in diverse mucocutaneous specimens. J Clin Microbiol. 2007; 45(4):1098-101. PMC: 1865822. DOI: 10.1128/JCM.02208-06. View

2.
Warren D, Liao R, Merz L, Eveland M, Dunne Jr W . Detection of methicillin-resistant Staphylococcus aureus directly from nasal swab specimens by a real-time PCR assay. J Clin Microbiol. 2004; 42(12):5578-81. PMC: 535250. DOI: 10.1128/JCM.42.12.5578-5581.2004. View

3.
Weist K, Cimbal A, Lecke C, Kampf G, Ruden H, Vonberg R . Evaluation of six agglutination tests for Staphylococcus aureus identification depending upon local prevalence of meticillin-resistant S. aureus (MRSA). J Med Microbiol. 2006; 55(Pt 3):283-290. DOI: 10.1099/jmm.0.46225-0. View

4.
Huletsky A, Giroux R, Rossbach V, Gagnon M, Vaillancourt M, Bernier M . New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci. J Clin Microbiol. 2004; 42(5):1875-84. PMC: 404602. DOI: 10.1128/JCM.42.5.1875-1884.2004. View

5.
Safdar N, Narans L, Gordon B, Maki D . Comparison of culture screening methods for detection of nasal carriage of methicillin-resistant Staphylococcus aureus: a prospective study comparing 32 methods. J Clin Microbiol. 2003; 41(7):3163-6. PMC: 165368. DOI: 10.1128/JCM.41.7.3163-3166.2003. View