Fluorescence in Situ Hybridization (FISH) on Maize Metaphase Chromosomes with Quantum Dot-labeled DNA Conjugates

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 2007 Nov 30

PMID 18046569

Citations 6

Authors

Lu Ma

Sheng-Mei Wu

Jing Huang

Yi Ding

Dai-Wen Pang

Lijia Li

Affiliations

Soon will be listed here.

Abstract

Semiconductor nanocrystals, also called quantum dots (QDs), are novel inorganic fluorophores which are brighter and more photostable than organic fluorophores. In the present study, highly dispersive QD-labeled oligonucleotide (TAG)(8) (QD-deoxyribonucleic acid [DNA]) conjugates were constructed via the metal-thiol bond, which can be used as fluorescence in situ hybridization (FISH) probes. FISH analysis of maize metaphase chromosomes using the QD-DNA probes showed that the probes could penetrate maize chromosomes and nuclei and solely hybridized to complementary target DNAs. Compared with the conventional organic dyes such as Cy3 and fluorescein isothiocyanate, this class of luminescent labels bound with oligonucleotides is brighter and more stable against photobleaching on the chromosomes after FISH. These results suggest that QD fluorophores may be a more stable and useful fluorescent label for FISH applications in plant chromosome mapping considering their size-tunable luminescence spectra.

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