Nanotechnology and Molecular Cytogenetics: the Future Has Not Yet Arrived

Overview

Journal Nano Rev

Specialty Biotechnology

Date 2011 Nov 24

PMID 22110858

Citations 10

Authors

Dimitris Ioannou

Darren K Griffin

Affiliations

Soon will be listed here.

Abstract

Quantum dots (QDs) are a novel class of inorganic fluorochromes composed of nanometer-scale crystals made of a semiconductor material. They are resistant to photo-bleaching, have narrow excitation and emission wavelengths that can be controlled by particle size and thus have the potential for multiplexing experiments. Given the remarkable optical properties that quantum dots possess, they have been proposed as an ideal material for use in molecular cytogenetics, specifically the technique of fluorescent in situ hybridisation (FISH). In this review, we provide an account of the current QD-FISH literature, and speculate as to why QDs are not yet optimised for FISH in their current form.

Citing Articles

Enhancing Optical and Electrical Performances via Nanocrystalline Si-Based Thin Films for Si Heterojunction Solar Cells.

Liang B, Chen X, Yuan H, Wang X, Hou G, Zhao Y ACS Omega. 2024; 9(50):49935-49944.

PMID: 39713640 PMC: 11656382. DOI: 10.1021/acsomega.4c09080.

Synthesis of Size-Adjustable CsPbBr Perovskite Quantum Dots for Potential Photoelectric Catalysis Applications.

Li H, He J, Wang X, Liu Q, Luo X, Wang M Materials (Basel). 2024; 17(7).

PMID: 38612121 PMC: 11012633. DOI: 10.3390/ma17071607.

Advances in Solution-Processed Blue Quantum Dot Light-Emitting Diodes.

Li S, Pan J, Yu Y, Zhao F, Wang Y, Liao L Nanomaterials (Basel). 2023; 13(10).

PMID: 37242111 PMC: 10222311. DOI: 10.3390/nano13101695.

Antibody Self-Assembly Maximizes Cytoplasmic Immunostaining Accuracy of Compact Quantum Dots.

Ma L, Geng J, Kolossov V, Han Z, Pei Y, Lim S Chem Mater. 2022; 33(13):4877-4889.

PMID: 35221487 PMC: 8880911. DOI: 10.1021/acs.chemmater.1c00164.

Telomere Distribution in Human Sperm Heads and Its Relation to Sperm Nuclear Morphology: A New Marker for Male Factor Infertility?.

Turner K, Watson E, Skinner B, Griffin D Int J Mol Sci. 2021; 22(14).

PMID: 34299219 PMC: 8306796. DOI: 10.3390/ijms22147599.

References

Rosenthal S, Tomlinson I, Adkins E, Schroeter S, Adams S, Swafford L . Targeting cell surface receptors with ligand-conjugated nanocrystals. J Am Chem Soc. 2002; 124(17):4586-94. DOI: 10.1021/ja003486s. View

Muller S, Cremer M, Neusser M, Grasser F, Cremer T . A technical note on quantum dots for multi-color fluorescence in situ hybridization. Cytogenet Genome Res. 2009; 124(3-4):351-9. DOI: 10.1159/000218138. View

Chan W, Nie S . Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science. 1998; 281(5385):2016-8. DOI: 10.1126/science.281.5385.2016. View

Rieger S, Kulkarni R, Darcy D, Fraser S, Koster R . Quantum dots are powerful multipurpose vital labeling agents in zebrafish embryos. Dev Dyn. 2005; 234(3):670-81. DOI: 10.1002/dvdy.20524. View

Dahan M, Levi S, Luccardini C, Rostaing P, Riveau B, Triller A . Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking. Science. 2003; 302(5644):442-5. DOI: 10.1126/science.1088525. View

Mansson A, Sundberg M, Balaz M, Bunk R, Nicholls I, Omling P . In vitro sliding of actin filaments labelled with single quantum dots. Biochem Biophys Res Commun. 2004; 314(2):529-34. DOI: 10.1016/j.bbrc.2003.12.133. View

Michalet X, Pinaud F, Bentolila L, Tsay J, Doose S, Li J . Quantum dots for live cells, in vivo imaging, and diagnostics. Science. 2005; 307(5709):538-44. PMC: 1201471. DOI: 10.1126/science.1104274. View

Mattheakis L, Dias J, Choi Y, Gong J, Bruchez M, Liu J . Optical coding of mammalian cells using semiconductor quantum dots. Anal Biochem. 2004; 327(2):200-8. DOI: 10.1016/j.ab.2004.01.031. View

Bruchez M . Turning all the lights on: quantum dots in cellular assays. Curr Opin Chem Biol. 2005; 9(5):533-7. DOI: 10.1016/j.cbpa.2005.08.019. View

10.

Resch-Genger U, Grabolle M, Cavaliere-Jaricot S, Nitschke R, Nann T . Quantum dots versus organic dyes as fluorescent labels. Nat Methods. 2008; 5(9):763-75. DOI: 10.1038/nmeth.1248. View

11.

Wu S, Zhao X, Zhang Z, Xie H, Tian Z, Peng J . Quantum-dot-labeled DNA probes for fluorescence in situ hybridization (FISH) in the microorganism Escherichia coli. Chemphyschem. 2006; 7(5):1062-7. DOI: 10.1002/cphc.200500608. View

12.

Zheng J, Ghazani A, Song Q, Mardyani S, Chan W, Wang C . Cellular imaging and surface marker labeling of hematopoietic cells using quantum dot bioconjugates. Lab Hematol. 2006; 12(2):94-8. DOI: 10.1532/LH96.04073. View

13.

Campos-Ramos R, Harvey S, Masabanda J, Carrasco L, Griffin D, McAndrew B . Identification of putative sex chromosomes in the blue tilapia, Oreochromis aureus, through synaptonemal complex and FISH analysis. Genetica. 2002; 111(1-3):143-53. DOI: 10.1023/a:1013707818534. View

14.

Ioannou D, Tempest H, Skinner B, Thornhill A, Ellis M, Griffin D . Quantum dots as new-generation fluorochromes for FISH: an appraisal. Chromosome Res. 2009; 17(4):519-30. DOI: 10.1007/s10577-009-9051-0. View

15.

Yao G, Wang L, Wu Y, Smith J, Xu J, Zhao W . FloDots: luminescent nanoparticles. Anal Bioanal Chem. 2006; 385(3):518-24. DOI: 10.1007/s00216-006-0452-z. View

16.

Sukhanova A, Devy J, Venteo L, Kaplan H, Artemyev M, Oleinikov V . Biocompatible fluorescent nanocrystals for immunolabeling of membrane proteins and cells. Anal Biochem. 2003; 324(1):60-7. DOI: 10.1016/j.ab.2003.09.031. View

17.

Alivisatos A, Gu W, Larabell C . Quantum dots as cellular probes. Annu Rev Biomed Eng. 2005; 7:55-76. DOI: 10.1146/annurev.bioeng.7.060804.100432. View

18.

Kang W, Chae J, Cho Y, Lee J, Kim S . Multiplex imaging of single tumor cells using quantum-dot-conjugated aptamers. Small. 2009; 5(22):2519-22. DOI: 10.1002/smll.200900848. View

19.

Finch K, Fonseka G, Ioannou D, Hickson N, Barclay Z, Chatzimeletiou K . Nuclear organisation in totipotent human nuclei and its relationship to chromosomal abnormality. J Cell Sci. 2008; 121(Pt 5):655-63. DOI: 10.1242/jcs.025205. View

20.

Mahmoud W, Sukhanova A, Oleinikov V, Rakovich Y, Donegan J, Pluot M . Emerging applications of fluorescent nanocrystals quantum dots for micrometastases detection. Proteomics. 2009; 10(4):700-16. DOI: 10.1002/pmic.200900540. View