TSG-6 Binds Via Its CUB_C Domain to the Cell-binding Domain of Fibronectin and Increases Fibronectin Matrix Assembly

Overview

Journal Matrix Biol

Publisher Elsevier

Specialties Biochemistry
Molecular Biology

Date 2007 Nov 29

PMID 18042364

Citations 25

Authors

Svetlana A Kuznetsova

David J Mahoney

Gema Martin-Manso

Tariq Ali

Hilke A Nentwich

John M Sipes

Bixi Zeng

Tikva Vogel

Anthony J Day

David D Roberts

Affiliations

Soon will be listed here.

Abstract

Human plasma fibronectin binds with high affinity to the inflammation-induced secreted protein TSG-6. Fibronectin binds to the CUB_C domain of TSG-6 but not to its Link module. TSG-6 can thus act as a bridging molecule to facilitate fibronectin association with the TSG-6 Link module ligand thrombospondin-1. Fibronectin binding to TSG-6 is divalent cation-independent and is conserved in cellular fibronectins. Based on competition binding studies using recombinant and proteolytic fragments of fibronectin, TSG-6 binding localizes to type III repeats 9-14 of fibronectin. This region of fibronectin contains the Arg-Gly-Asp sequence recognized by alpha5beta1 integrin, but deletion of that sequence does not prevent TSG-6 binding, and TSG-6 does not inhibit cell adhesion on fibronectin substrates mediated by this integrin. This region of fibronectin is also involved in fibronectin matrix assembly, and addition of TSG-6 enhances exogenous and endogenous fibronectin matrix assembly by human fibroblasts. Therefore, TSG-6 is a high affinity ligand that can mediate fibronectin interactions with other matrix components and modulate some interactions of fibronectin with cells.

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