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Rapid Determination of the Three-dimensional Orientation of Single Molecules

Overview
Journal Opt Lett
Specialty Ophthalmology
Date 2007 Nov 23
PMID 18033550
Citations 38
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Abstract

A technique is proposed for determination of the three-dimensional orientation of the transition dipoles of single molecules by use of polarization-sensitive detection of fluorescence through a high-N.A. objective. Molecular orientation can be determined uniquely and rapidly based on the counts from three detectors that are sensitive to different polarizations of light.

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