Visualization of Proteases Within a Complex Sample Following Their Selective Retention on Immobilized Bacitracin, a Peptide Antibiotic

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 1991 Nov 1

PMID 1799226

Citations 4

Authors

J M van Noort

P van den Berg

I E MATTERN

Affiliations

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Abstract

A method for the visualization of individual proteases within a complex biological sample is described. In a single chromatographic step, proteases can be separated from other biomolecules by selective binding to immobilized bacitracin, a peptide antibiotic. Following desorption, proteases may be separated by SDS-polyacrylamide gel electrophoresis. The application of this method is presented in the visualization of proteases secreted by the fungus Aspergillus niger.

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