Intracellular Trafficking Pathway of BK Virus in Human Renal Proximal Tubular Epithelial Cells

Overview

Journal Virology

Specialty Microbiology

Date 2007 Nov 3

PMID 17976677

Citations 26

Authors

Takahito Moriyama

Andrey Sorokin

Affiliations

Soon will be listed here.

Abstract

Intracellular trafficking of BK Virus (BKV) in human renal proximal tubular epithelial cells (HRPTEC) is critical for BKV nephritis. However, the major trafficking components utilized by BKV remain unknown. Coincubation of HRPTEC with BKV and microtubule disrupting agents prevented BKV infection as detected by immunofluorescence and western blot analysis with antibodies which recognize BKV large T antigen. However, inhibition of a dynein, cellular motor protein, did not interfere with BKV infection in HRPTEC. A colocalization study of BKV with the markers of the endoplasmic reticulum (ER) and the Golgi apparatus (GA), indicated that BKV reached the ER from 6 to 10 h, while bypassing the GA or passing through the GA too transiently to be detected. This study contributes to the understanding of mechanisms of intracellular trafficking used by BKV in the infection of HRPTEC.

Citing Articles

Massive entry of BK Polyomavirus induces transient cytoplasmic vacuolization of human renal proximal tubule epithelial cells.

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Bruschi M, Granata S, Candiano G, Petretto A, Bartolucci M, Ghiggeri G Front Med (Lausanne). 2022; 9:1028085.

PMID: 36465937 PMC: 9712214. DOI: 10.3389/fmed.2022.1028085.

Acitretin and Retinoic Acid Derivatives Inhibit BK Polyomavirus Replication in Primary Human Proximal Renal Tubular Epithelial and Urothelial Cells.

Wu Z, Graf F, Hirsch H J Virol. 2021; 95(15):e0012721.

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Non-permissive human conventional CD1c+ dendritic cells enable trans-infection of human primary renal tubular epithelial cells and protect BK polyomavirus from neutralization.

Sikorski M, Coulon F, Peltier C, Braudeau C, Garcia A, Giraud M PLoS Pathog. 2021; 17(2):e1009042.

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