Cell Penetration and Trafficking of Polyomavirus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2003 Jan 29

PMID 12552000

Citations 32

Authors

Joanna M Gilbert

Ilya G Goldberg

Thomas L Benjamin

Affiliations

Soon will be listed here.

Abstract

The murine polyomavirus (Py) enters mouse fibroblasts and kidney epithelial cells via an endocytic pathway that is caveola-independent (as well as clathrin-independent). In contrast, uptake of simian virus 40 into the same cells is dependent on caveola. Following the initial uptake of Py, both microtubules and microfilaments play roles in trafficking of the virus to the nucleus. Colcemid, which disrupts microtubules, inhibits the ability of Py to reach the nucleus and replicate. Paclitaxel, which stabilizes microtubules and prevents microtubule turnover, has no effect, indicating that intact but not dynamic microtubules are required for Py infectivity. Compounds that disrupt actin filaments enhance Py uptake while stabilization of actin filaments impedes Py infection. Virus particles are seen in association with actin in cells treated with microfilament-disrupting or filament-stabilizing agents at levels comparable to those in untreated cells, suggesting that a dynamic state of the microfilament system is important for Py infectivity.

Citing Articles

Microtubules in Polyomavirus Infection.

Hornikova L, Brustikova K, Forstova J Viruses. 2020; 12(1).

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Identification of Rab18 as an Essential Host Factor for BK Polyomavirus Infection Using a Whole-Genome RNA Interference Screen.

Zhao L, Imperiale M mSphere. 2017; 2(4).

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Murine Polyomavirus Cell Surface Receptors Activate Distinct Signaling Pathways Required for Infection.

OHara S, Garcea R mBio. 2016; 7(6).

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Caveolin- and clathrin-independent entry of BKPyV into primary human proximal tubule epithelial cells.

Zhao L, Marciano A, Rivet C, Imperiale M Virology. 2016; 492:66-72.

PMID: 26901486 PMC: 5051663. DOI: 10.1016/j.virol.2016.02.007.

Coat as a dagger: the use of capsid proteins to perforate membranes during non-enveloped DNA viruses trafficking.

Bilkova E, Forstova J, Abrahamyan L Viruses. 2014; 6(7):2899-937.

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