A Gene Regulatory Network in Mouse Embryonic Stem Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2007 Oct 18

PMID 17940043

Citations 126

Authors

Qing Zhou

Hiram Chipperfield

Douglas A Melton

Wing Hung Wong

Affiliations

Soon will be listed here.

Abstract

We analyze new and existing expression and transcription factor-binding data to characterize gene regulatory relations in mouse ES cells (ESC). In addition to confirming the key roles of Oct4, Sox2, and Nanog, our analysis identifies several genes, such as Esrrb, Stat3, Tcf7, Sall4, and LRH-1, as statistically significant coregulators. The regulatory interactions among 15 core regulators are used to construct a gene regulatory network in ESC. The network encapsulates extensive cross-regulations among the core regulators, highlights how they may control epigenetic processes, and reveals the surprising roles of nuclear receptors. Our analysis also provides information on the regulation of a large number of putative target genes of the network.

Citing Articles

Approaches for Benchmarking Single-Cell Gene Regulatory Network Methods.

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Sall4 and Gata4 induce cardiac fibroblast transition towards a partially multipotent state with cardiogenic potential.

Gao H, Pathan S, Dixon B, Pugazenthi A, Mathison M, Mohamed T Sci Rep. 2024; 14(1):24182.

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A suboptimal OCT4-SOX2 binding site facilitates the naïve-state specific function of a Klf4 enhancer.

Waite J, Boytz R, Traeger A, Lind T, Lumbao-Conradson K, Torigoe S PLoS One. 2024; 19(9):e0311120.

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Identification of an embryonic differentiation stage marked by Sox1 and FoxA2 co-expression using combined cell tracking and high dimensional protein imaging.

Arekatla G, Skylaki S, Corredor Suarez D, Jackson H, Schapiro D, Engler S Nat Commun. 2024; 15(1):7860.

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Sall4 regulates downstream patterning genes during limb regeneration.

Erickson J, Walker S, Arenas Gomez C, Echeverri K Dev Biol. 2024; 515:151-159.

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