Genetic Relatedness of Commensal Strains of Candida Albicans Carried in the Oral Cavity of Patients' Dental Prosthesis Users in Brazil

Overview

Journal Mycopathologia

Specialties Microbiology
Pharmacology

Date 2007 Oct 2

PMID 17906942

Citations 8

Authors

Regina Helena Pires-Goncalves

Elaine Toscano Miranda

Lilian Cristiane Baeza

Marcelo Teruyuki Matsumoto

Jose Eduardo Zaia

Maria Jose Soares Mendes-Giannini

Affiliations

Soon will be listed here.

Abstract

The aim of this study is to describe the degree of yeast-colonization in diabetic and hemodialysed-users of dental prostheses. Individuals (306) were examined using an oral rinse technique in order to evaluate the incidence of yeast-carriage, and genotype of C. albicans. Yeasts were isolated from 68.4% (91/133) individual's dental prostheses users. Dental prostheses were found to be a significant factor for the yeast colonization (P < 0.05). Overall, the intensity of carriage was higher in diabetic patients as compared with health and hemodialysed individuals (P < 0.05). The isolation rates were: C. albicans (51.7%), C. parapsilosis (20.9%), C. tropicalis (14.3%), C. glabrata (6.6%), C. krusei (3.3%), C. rugosa (1.1%), and Pichia (Pichia ohmeri, 2.2%). Ready-To-Go RAPD Analysis Beads were used and primer OPJ 6 distinguished the C. albicans isolates found in prostheses users. All the isolates were grouped into 11 RAPD profiles in four main clusters and, the average S (AB) for the entire collection of 47 C. albicans isolates were 0.779 +/- 0.178. Over 85% of isolates had a similarity level higher than or equal to 0.8 reinforcing the idea that the use of dental prostheses, independently of the host's clinical condition, probably provides the necessary conditions for these strains to gain a growth-specific advantage over others.

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References

Cannon R, Chaffin W . Colonization is a crucial factor in oral candidiasis. J Dent Educ. 2001; 65(8):785-7. View

Goncalves R, Miranda E, Zaia J, Mendes Giannini M . Species diversity of yeast in oral colonization of insulin-treated diabetes mellitus patients. Mycopathologia. 2006; 162(2):83-9. DOI: 10.1007/s11046-006-0038-5. View

Gales A, Pfaller M, Houston A, Joly S, Sullivan D, Coleman D . Identification of Candida dubliniensis based on temperature and utilization of xylose and alpha-methyl-D-glucoside as determined with the API 20C AUX and vitek YBC systems. J Clin Microbiol. 1999; 37(12):3804-8. PMC: 85818. DOI: 10.1128/JCM.37.12.3804-3808.1999. View

Xu J, Vilgalys R, Mitchell T . Lack of genetic differentiation between two geographically diverse samples of Candida albicans isolated from patients infected with human immunodeficiency virus. J Bacteriol. 1999; 181(4):1369-73. PMC: 93522. DOI: 10.1128/JB.181.4.1369-1373.1999. View

Hossain H, Ansari F, Schulz-Weidner N, Wetzel W, Chakraborty T, Domann E . Clonal identity of Candida albicans in the oral cavity and the gastrointestinal tract of pre-school children. Oral Microbiol Immunol. 2003; 18(5):302-8. DOI: 10.1034/j.1399-302x.2003.00086.x. View

Narhi T, Ainamo A, Meurman J . Salivary yeasts, saliva, and oral mucosa in the elderly. J Dent Res. 1993; 72(6):1009-14. DOI: 10.1177/00220345930720060301. View

Schmid J, Rotman M, Reed B, Pierson C, Soll D . Genetic similarity of Candida albicans strains from vaginitis patients and their partners. J Clin Microbiol. 1993; 31(1):39-46. PMC: 262617. DOI: 10.1128/jcm.31.1.39-46.1993. View

Budtz-Jorgensen E, STENDERUP A, Grabowski M . An epidemiologic study of yeasts in elderly denture wearers. Community Dent Oral Epidemiol. 1975; 3(3):115-9. DOI: 10.1111/j.1600-0528.1975.tb00291.x. View

Abu-Elteen K . The prevalence of Candida albicans populations in the mouths of complete denture wearers. New Microbiol. 1998; 21(1):41-8. View

10.

Matsumoto M, Fusco-Almeida A, Baeza L, de Souza Carvalho Melhem M, Medes-Giannini M . Genotyping, serotyping and determination of mating-type of Cryptococcus neoformans clinical isolates from São Paulo State, Brazil. Rev Inst Med Trop Sao Paulo. 2007; 49(1):41-7. DOI: 10.1590/s0036-46652007000100008. View

11.

Soysa N, Samaranayake L, Ellepola A . Diabetes mellitus as a contributory factor in oral candidosis. Diabet Med. 2006; 23(5):455-9. DOI: 10.1111/j.1464-5491.2005.01701.x. View

12.

Samaranayake L, MacFarlane T, Lamey P, Ferguson M . A comparison of oral rinse and imprint sampling techniques for the detection of yeast, coliform and Staphylococcus aureus carriage in the oral cavity. J Oral Pathol. 1986; 15(7):386-8. DOI: 10.1111/j.1600-0714.1986.tb00646.x. View

13.

Al-Karaawi Z, Manfredi M, Waugh A, McCullough M, Jorge J, Scully C . Molecular characterization of Candida spp. isolated from the oral cavities of patients from diverse clinical settings. Oral Microbiol Immunol. 2002; 17(1):44-9. DOI: 10.1046/j.0902-0055.2001.00081.x. View

14.

Kleinegger C, Lockhart S, Vargas K, Soll D . Frequency, intensity, species, and strains of oral Candida vary as a function of host age. J Clin Microbiol. 1996; 34(9):2246-54. PMC: 229226. DOI: 10.1128/jcm.34.9.2246-2254.1996. View

15.

Chong P, Lee Y, Tan B, Ng K . Genetic relatedness of Candida strains isolated from women with vaginal candidiasis in Malaysia. J Med Microbiol. 2003; 52(Pt 8):657-666. DOI: 10.1099/jmm.0.04973-0. View

16.

Ghannoum M, Abu-Elteen K . Pathogenicity determinants of Candida. Mycoses. 1990; 33(6):265-82. DOI: 10.1111/myc.1990.33.6.265. View

17.

Xu J, Boyd C, Livingston E, Meyer W, Madden J, Mitchell T . Species and genotypic diversities and similarities of pathogenic yeasts colonizing women. J Clin Microbiol. 1999; 37(12):3835-43. PMC: 85824. DOI: 10.1128/JCM.37.12.3835-3843.1999. View

18.

Barbeau J, Seguin J, Goulet J, de Koninck L, Avon S, Lalonde B . Reassessing the presence of Candida albicans in denture-related stomatitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 95(1):51-9. DOI: 10.1067/moe.2003.44. View