Effects of Quercetin on Mushroom Tyrosinase and B16-F10 Melanoma Cells

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2007 Sep 18

PMID 17873839

Citations 14

Authors

Isao Kubo

Teruhiko Nitoda

Ken-Ichi Nihei

Affiliations

Soon will be listed here.

Abstract

In searching for tyrosinase inhibitors from plants using L-3,4-dihydroxyphenylalanine (L-DOPA) as a substrate, quercetin was found to be partially oxidized to the corresponding o-quinone under catalysis by mushroom tyrosinase (EC 1.14.18.1). Simultaneously, L-DOPA was also oxidized to dopaquinone and both o-quinones were further oxidized, respectively. The remaining quercetin partially formed adducts with dopaquinone through a Michael type addition. In general, flavonols form adducts with dopaquinone as long as their 3-hydroxyl group is free. Quercetin enhanced melanin production per cell in cultured murine B16-F10 melanoma cells, but this effect may be due in part to melanocytotoxicity. The concentration leading to 50% viable cells lost was established as 20 microM and almost complete lethality was observed at 80 microM.

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