Alpha 2.0
Login Register
» Articles » PMID: 1785941

Purification and Characterization of the N-methylcarbamate Hydrolase from Pseudomonas Strain CRL-OK

Overview
Journal Appl Environ Microbiol
Specialties Environmental Health
Microbiology
Date 1991 Dec 1
PMID 1785941
Citations 12
Authors
W W Mulbry
R W Eaton
Affiliations
Soon will be listed here.
Abstract

A unique cytosolic enzyme that hydrolyzes the carbamate linkage of the insecticide carbaryl (1-naphthyl N-methylcarbamate) was purified from extracts of Pseudomonas sp. strain CRL-OK. Substrates of the hydrolase include the N-methylcarbamate pesticides carbofuran and aldicarb but not the phenylcarbamate isopropyl m-chlorocarbanilate, the thiocarbamate S-ethyl N,N-dipropylthiocarbamate, or the dimethylcarbamate o-nitrophenyldimethylcarbamate.

Citing Articles

Comparative Genomic Analysis of Carbofuran-Degrading Sphingomonads Reveals the Carbofuran Catabolism Mechanism in sp. Strain CFD-1.

Jiang W, Zhang M, Gao S, Zhu Q, Qiu J, Yan X Appl Environ Microbiol. 2022; 88(22):e0102422.

PMID: 36314801 PMC: 9680625. DOI: 10.1128/aem.01024-22.

Carbaryl as a Carbon and Nitrogen Source: an Inducible Methylamine Metabolic Pathway at the Biochemical and Molecular Levels in sp. Strain C5pp.

Kamini , Sharma R, Punekar N, Phale P Appl Environ Microbiol. 2018; 84(24).

PMID: 30315077 PMC: 6275335. DOI: 10.1128/AEM.01866-18.

Biodegradation of chlorpropham and its major products by Bacillus licheniformis NKC-1.

Pujar N, Premakshi H, Laad S, Pattar S, Mirjankar M, Kamanavalli C World J Microbiol Biotechnol. 2018; 34(8):112.

PMID: 29980862 DOI: 10.1007/s11274-018-2494-8.

Discovery of carbamate degrading enzymes by functional metagenomics.

Ufarte L, Laville E, Duquesne S, Morgavi D, Robe P, Klopp C PLoS One. 2017; 12(12):e0189201.

PMID: 29240834 PMC: 5730166. DOI: 10.1371/journal.pone.0189201.

Compartmentalization of the Carbaryl Degradation Pathway: Molecular Characterization of Inducible Periplasmic Carbaryl Hydrolase from Pseudomonas spp.

Kamini , Shetty D, Trivedi V, Varunjikar M, Phale P Appl Environ Microbiol. 2017; 84(2).

PMID: 29079626 PMC: 5752863. DOI: 10.1128/AEM.02115-17.

References
1.
Chapalamadugu S, Chaudhry G . Hydrolysis of carbaryl by a Pseudomonas sp. and construction of a microbial consortium that completely metabolizes carbaryl. Appl Environ Microbiol. 1991; 57(3):744-50. PMC: 182789. DOI: 10.1128/aem.57.3.744-750.1991. View
2.
Chaudhry G, Ali A . Bacterial metabolism of carbofuran. Appl Environ Microbiol. 1988; 54(6):1414-9. PMC: 202671. DOI: 10.1128/aem.54.6.1414-1419.1988. View
3.
Larkin M, Day M . The metabolism of carbaryl by three bacterial isolates, Pseudomonas spp. (NCIB 12042 & 12043) and Rhodococcus sp. (NCIB 12038) from garden soil. J Appl Bacteriol. 1986; 60(3):233-42. DOI: 10.1111/j.1365-2672.1986.tb01078.x. View
4.
Mulbry W, Karns J . Purification and characterization of three parathion hydrolases from gram-negative bacterial strains. Appl Environ Microbiol. 1989; 55(2):289-93. PMC: 184103. DOI: 10.1128/aem.55.2.289-293.1989. View
5.
Ahmad N, Walgenbach D, Sutter G . Degradation rates of technical carbofuran and a granular formulation in four soils with known insecticide use history. Bull Environ Contam Toxicol. 1979; 23(4-5):572-4. DOI: 10.1007/BF01770005. View