Nucleotide Sequence and Genetic Structure of a Novel Carbaryl Hydrolase Gene (cehA) from Rhizobium Sp. Strain AC100

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2002 Mar 2

PMID 11872471

Citations 24

Authors

Masayuki Hashimoto

Mitsuru Fukui

Kouichi Hayano

Masahito Hayatsu

Affiliations

Soon will be listed here.

Abstract

Rhizobium sp. strain AC100, which is capable of degrading carbaryl (1-naphthyl-N-methylcarbamate), was isolated from soil treated with carbaryl. This bacterium hydrolyzed carbaryl to 1-naphthol and methylamine. Carbaryl hydrolase from the strain was purified to homogeneity, and its N-terminal sequence, molecular mass (82 kDa), and enzymatic properties were determined. The purified enzyme hydrolyzed 1-naphthyl acetate and 4-nitrophenyl acetate indicating that the enzyme is an esterase. We then cloned the carbaryl hydrolase gene (cehA) from the plasmid DNA of the strain and determined the nucleotide sequence of the 10-kb region containing cehA. No homologous sequences were found by a database homology search using the nucleotide and deduced amino acid sequences of the cehA gene. Six open reading frames including the cehA gene were found in the 10-kb region, and sequencing analysis shows that the cehA gene is flanked by two copies of insertion sequence-like sequence, suggesting that it makes part of a composite transposon.

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References

Xu Y, Mortimer M, Fisher T, Kahn M, Brockman F, Xun L . Cloning, sequencing, and analysis of a gene cluster from Chelatobacter heintzii ATCC 29600 encoding nitrilotriacetate monooxygenase and NADH:flavin mononucleotide oxidoreductase. J Bacteriol. 1997; 179(4):1112-6. PMC: 178806. DOI: 10.1128/jb.179.4.1112-1116.1997. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Hanahan D . Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983; 166(4):557-80. DOI: 10.1016/s0022-2836(83)80284-8. View

Ditta G, Stanfield S, Corbin D, Helinski D . Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980; 77(12):7347-51. PMC: 350500. DOI: 10.1073/pnas.77.12.7347. View

Ogawa N, Miyashita K . Recombination of a 3-chlorobenzoate catabolic plasmid from Alcaligenes eutrophus NH9 mediated by direct repeat elements. Appl Environ Microbiol. 1995; 61(11):3788-95. PMC: 167680. DOI: 10.1128/aem.61.11.3788-3795.1995. View

Ogramab , Duana , Trabuea1 , Fengc2 , Castroa , Oua . Carbofuran degradation mediated by three related plasmid systems. FEMS Microbiol Ecol. 2000; 32(3):197-203. DOI: 10.1111/j.1574-6941.2000.tb00712.x. View

Brown H, Stokes H, Hall R . The integrons In0, In2, and In5 are defective transposon derivatives. J Bacteriol. 1996; 178(15):4429-37. PMC: 178208. DOI: 10.1128/jb.178.15.4429-4437.1996. View

Stover C, Pham X, Erwin A, Mizoguchi S, Warrener P, Hickey M . Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature. 2000; 406(6799):959-64. DOI: 10.1038/35023079. View

Desaint , Hartmann , Parekh , Fournier . Genetic diversity of carbofuran-degrading soil bacteria. FEMS Microbiol Ecol. 2000; 34(2):173-180. DOI: 10.1111/j.1574-6941.2000.tb00767.x. View

10.

Karpouzas D, Morgan J, Walker A . Isolation and characterization of 23 carbofuran-degrading bacteria from soils from distant geographical areas. Lett Appl Microbiol. 2000; 31(5):353-8. DOI: 10.1046/j.1472-765x.2000.00823.x. View

11.

FAHMY M, Fukuto T, Myers R, MARCH R . The selective toxicity of new N-phosphorothioyl-carbamate esters. J Agric Food Chem. 1970; 18(5):793-6. DOI: 10.1021/jf60171a014. View

12.

Felsot A, Maddox J, Bruce W . Enhanced microbial degradation of carbofuran in soils with histories of Furadan use. Bull Environ Contam Toxicol. 1981; 26(6):781-8. DOI: 10.1007/BF01622171. View

13.

Chaudhry G, Ali A . Bacterial metabolism of carbofuran. Appl Environ Microbiol. 1988; 54(6):1414-9. PMC: 202671. DOI: 10.1128/aem.54.6.1414-1419.1988. View

14.

Somers E, Keijers V, Ptacek D, Halvorsen Ottoy M, Srinivasan M, Vanderleyden J . The salCAB operon of Azospirillum irakense, required for growth on salicin, is repressed by SalR, a transcriptional regulator that belongs to the Lacl/GalR family. Mol Gen Genet. 2000; 263(6):1038-46. DOI: 10.1007/pl00008692. View

15.

Rogers M, Bennett T, Payne C, Smith C . Insertional activation of cepA leads to high-level beta-lactamase expression in Bacteroides fragilis clinical isolates. J Bacteriol. 1994; 176(14):4376-84. PMC: 205651. DOI: 10.1128/jb.176.14.4376-4384.1994. View

16.

Figurski D, Helinski D . Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci U S A. 1979; 76(4):1648-52. PMC: 383447. DOI: 10.1073/pnas.76.4.1648. View

17.

Chapalamadugu S, Chaudhry G . Microbiological and biotechnological aspects of metabolism of carbamates and organophosphates. Crit Rev Biotechnol. 1992; 12(5-6):357-89. DOI: 10.3109/07388559209114232. View

18.

Feng X, Ou L, Ogram A . Plasmid-mediated mineralization of carbofuran by Sphingomonas sp. strain CF06. Appl Environ Microbiol. 1997; 63(4):1332-7. PMC: 168426. DOI: 10.1128/aem.63.4.1332-1337.1997. View

19.

Hansen J, Olsen R . Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5. J Bacteriol. 1978; 135(1):227-38. PMC: 224811. DOI: 10.1128/jb.135.1.227-238.1978. View

20.

Ogawa N, Miyashita K . The chlorocatechol-catabolic transposon Tn5707 of Alcaligenes eutrophus NH9, carrying a gene cluster highly homologous to that in the 1,2,4-trichlorobenzene-degrading bacterium Pseudomonas sp. strain P51, confers the ability to grow on.... Appl Environ Microbiol. 1999; 65(2):724-31. PMC: 91086. DOI: 10.1128/AEM.65.2.724-731.1999. View