Centrosomal Pre-integration Latency of HIV-1 in Quiescent Cells

Overview

Journal Retrovirology

Publisher Biomed Central

Specialty Microbiology

Date 2007 Sep 12

PMID 17845727

Citations 25

Authors

Alessia Zamborlini

Jacqueline Lehmann-Che

Emmanuel Clave

Marie-Lou Giron

Joelle Tobaly-Tapiero

Philippe Roingeard

Stephane Emiliani

Antoine Toubert

Hugues de The

Ali Saib

Affiliations

Soon will be listed here.

Abstract

Human immunodeficiency virus type 1 (HIV-1) efficiently replicates in dividing and non-dividing cells. However, HIV-1 infection is blocked at an early post-entry step in quiescent CD4+ T cells in vitro. The molecular basis of this restriction is still poorly understood. Here, we show that in quiescent cells, incoming HIV-1 sub-viral complexes concentrate and stably reside at the centrosome for several weeks. Upon cell activation, viral replication resumes leading to viral gene expression. Thus, HIV-1 can persist in quiescent cells as a stable, centrosome-associated, pre-integration intermediate.

Citing Articles

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References

Nigg E . Centrosome duplication: of rules and licenses. Trends Cell Biol. 2007; 17(5):215-21. DOI: 10.1016/j.tcb.2007.03.003. View

Ganesh L, Burstein E, Guha-Niyogi A, Louder M, Mascola J, Klomp L . The gene product Murr1 restricts HIV-1 replication in resting CD4+ lymphocytes. Nature. 2003; 426(6968):853-7. DOI: 10.1038/nature02171. View

Swiggard W, ODoherty U, McGain D, Jeyakumar D, Malim M . Long HIV type 1 reverse transcripts can accumulate stably within resting CD4+ T cells while short ones are degraded. AIDS Res Hum Retroviruses. 2004; 20(3):285-95. DOI: 10.1089/088922204322996527. View

Petit C, Giron M, Tobaly-Tapiero J, Bittoun P, Real E, Jacob Y . Targeting of incoming retroviral Gag to the centrosome involves a direct interaction with the dynein light chain 8. J Cell Sci. 2003; 116(Pt 16):3433-42. DOI: 10.1242/jcs.00613. View

McDonald D, Vodicka M, Lucero G, Svitkina T, Borisy G, Emerman M . Visualization of the intracellular behavior of HIV in living cells. J Cell Biol. 2002; 159(3):441-52. PMC: 2173076. DOI: 10.1083/jcb.200203150. View

Pierson T, Zhou Y, Kieffer T, Ruff C, Buck C, Siliciano R . Molecular characterization of preintegration latency in human immunodeficiency virus type 1 infection. J Virol. 2002; 76(17):8518-31. PMC: 136977. DOI: 10.1128/jvi.76.17.8518-8513.2002. View

Eckstein D, Penn M, Korin Y, Zack J, Kreisberg J, Roederer M . HIV-1 actively replicates in naive CD4(+) T cells residing within human lymphoid tissues. Immunity. 2001; 15(4):671-82. DOI: 10.1016/s1074-7613(01)00217-5. View

Khodjakov A, Rieder C . Centrosomes enhance the fidelity of cytokinesis in vertebrates and are required for cell cycle progression. J Cell Biol. 2001; 153(1):237-42. PMC: 2185537. DOI: 10.1083/jcb.153.1.237. View

Hinchcliffe E, Miller F, Cham M, Khodjakov A, Sluder G . Requirement of a centrosomal activity for cell cycle progression through G1 into S phase. Science. 2001; 291(5508):1547-50. DOI: 10.1126/science.1056866. View

10.

Ploubidou A, Way M . Viral transport and the cytoskeleton. Curr Opin Cell Biol. 2001; 13(1):97-105. PMC: 7125730. DOI: 10.1016/s0955-0674(00)00180-0. View

11.

ODoherty U, Swiggard W, Malim M . Human immunodeficiency virus type 1 spinoculation enhances infection through virus binding. J Virol. 2000; 74(21):10074-80. PMC: 102046. DOI: 10.1128/jvi.74.21.10074-10080.2000. View

12.

Zhang H, Dornadula G, Orenstein J, Pomerantz R . Morphologic changes in human immunodeficiency virus type 1 virions secondary to intravirion reverse transcription: evidence indicating that reverse transcription may not take place within the intact viral core. J Hum Virol. 2000; 3(3):165-72. View

13.

Lehmann-Che J, Renault N, Giron M, Roingeard P, Clave E, Tobaly-Tapiero J . Centrosomal latency of incoming foamy viruses in resting cells. PLoS Pathog. 2007; 3(5):e74. PMC: 1871606. DOI: 10.1371/journal.ppat.0030074. View

14.

Arhel N, Souquere-Besse S, Munier S, Souque P, Guadagnini S, Rutherford S . HIV-1 DNA Flap formation promotes uncoating of the pre-integration complex at the nuclear pore. EMBO J. 2007; 26(12):3025-37. PMC: 1894778. DOI: 10.1038/sj.emboj.7601740. View

15.

Suzuki Y, Craigie R . The road to chromatin - nuclear entry of retroviruses. Nat Rev Microbiol. 2007; 5(3):187-96. DOI: 10.1038/nrmicro1579. View

16.

Raynaud-Messina B, Merdes A . Gamma-tubulin complexes and microtubule organization. Curr Opin Cell Biol. 2006; 19(1):24-30. DOI: 10.1016/j.ceb.2006.12.008. View

17.

Fackler O, Krausslich H . Interactions of human retroviruses with the host cell cytoskeleton. Curr Opin Microbiol. 2006; 9(4):409-15. DOI: 10.1016/j.mib.2006.06.010. View

18.

Yamashita M, Emerman M . The cell cycle independence of HIV infections is not determined by known karyophilic viral elements. PLoS Pathog. 2005; 1(3):e18. PMC: 1283251. DOI: 10.1371/journal.ppat.0010018. View

19.

Kreisberg J, Yonemoto W, Greene W . Endogenous factors enhance HIV infection of tissue naive CD4 T cells by stimulating high molecular mass APOBEC3G complex formation. J Exp Med. 2006; 203(4):865-70. PMC: 2118295. DOI: 10.1084/jem.20051856. View

20.

Yamashita M, Emerman M . Retroviral infection of non-dividing cells: old and new perspectives. Virology. 2005; 344(1):88-93. DOI: 10.1016/j.virol.2005.09.012. View