JBP2, a SWI2/SNF2-like Protein, Regulates De Novo Telomeric DNA Glycosylation in Bloodstream Form Trypanosoma Brucei

Overview

Journal Mol Biochem Parasitol

Specialties Biochemistry
Molecular Biology
Parasitology

Date 2007 Aug 21

PMID 17706299

Citations 17

Authors

Rudo Kieft

Verena Brand

Dilrukshi K Ekanayake

Kate Sweeney

Courtney DiPaolo

William S Reznikoff

Robert Sabatini

Affiliations

Soon will be listed here.

Abstract

Synthesis of the modified thymine base, beta-d-glucosyl-hydroxymethyluracil or J, within telomeric DNA of Trypanosoma brucei correlates with the bloodstream form specific epigenetic silencing of telomeric variant surface glycoprotein genes involved in antigenic variation. In order to analyze the function of base J in the regulation of antigenic variation, we are characterizing the regulatory mechanism of J biosynthesis. We have recently proposed a model in which chromatin remodeling by a SWI2/SNF2-like protein (JBP2) regulates the developmental and de novo site-specific localization of J synthesis within bloodstream form trypanosome DNA. Consistent with this model, we now show that JBP2 (-/-) bloodstream form trypanosomes contain five-fold less base J and are unable to stimulate de novo J synthesis in newly generated telomeric arrays.

Citing Articles

Behind Base J: The Roles of JBP1 and JBP2 on Trypanosomatids.

Assis L, de Paiva S, Cano M Pathogens. 2023; 12(3).

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The SPARC complex defines RNAPII promoters in .

Staneva D, Bresson S, Auchynnikava T, Spanos C, Rappsilber J, Jeyaprakash A Elife. 2022; 11.

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Carbohydrate biomarkers for future disease detection and treatment.

Cheng Y, Li M, Wang S, Peng H, Reid S, Ni N Sci China Chem. 2020; 53(1):3-20.

PMID: 32214994 PMC: 7089153. DOI: 10.1007/s11426-010-0021-3.

Identification of a novel base J binding protein complex involved in RNA polymerase II transcription termination in trypanosomes.

Kieft R, Zhang Y, Marand A, Moran J, Bridger R, Wells L PLoS Genet. 2020; 16(2):e1008390.

PMID: 32084124 PMC: 7055916. DOI: 10.1371/journal.pgen.1008390.

A method for the efficient and selective identification of 5-hydroxymethyluracil in genomic DNA.

Bullard W, Kieft R, Sabatini R Biol Methods Protoc. 2017; 2(1).

PMID: 29276783 PMC: 5741180. DOI: 10.1093/biomethods/bpw006.

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