A Novel Role for Connexin Hemichannel in Oxidative Stress and Smoking-induced Cell Injury

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2007 Aug 9

PMID 17684558

Citations 69

Authors

Srinivasan Ramachandran

Lai-Hua Xie

Scott A John

Shankar Subramaniam

Ratnesh Lal

Affiliations

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Abstract

Oxidative stress is linked to many pathological conditions, including ischemia, atherosclerosis and neurodegenerative disorders. The molecular mechanisms of oxidative stress induced pathophysiology and cell death are currently poorly understood. Our present work demonstrates that oxidative stress induced by reactive oxygen species and cigarette smoke extract depolarize the cell membrane and open connexin hemichannels. Under oxidative stress, connexin expression and connexin silencing resulted in increased and reduced cell deaths, respectively. Morphological and live/dead assays indicate that cell death is likely through apoptosis. Our studies provide new insights into the mechanistic role of hemichannels in oxidative stress induced cell injury.

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