Assessment Using Human Dental Pulp Cells of Clastogenicity of Antiseptics Used in Dental Practice and Agents for Root Canal Enlargement and Cleaning

Overview

Journal Odontology

Specialty Dentistry

Date 2007 Jul 31

PMID 17660979

Citations 1

Authors

Itsuro Hori

Yukari Higo

Maki Ohno

Takeo W Tsutsui

Takeki Tsutsui

Affiliations

Soon will be listed here.

Abstract

Numerous and varied chemical agents are used as topically applied drugs in dental practice. As they are administered directly to the oral cavity, it is important to study the safety of these agents. In the present study, to assess safety regarding mutagenicity, we investigated the abilities of six antiseptics to induce chromosome aberrations in human dental pulp cells. The antiseptics tested were benzalkonium chloride, benzethonium chloride, iodine glycerin, iodine tincture, oxydol, and povidone-iodine. In addition, we tested two agents used for root canal enlargement and cleaning, ethylenediaminetetraacetic acid and sodium hypochlorite. Chromosome aberrations were induced only in cells treated with the highest concentration of iodine tincture for 30 h. The other chemical agents failed to induce chromosome aberrations in the presence or absence of exogenous metabolic activation. The concentration of iodine tincture to which patients are exposed in dental practice is 1000-fold the concentration that induced chromosome aberrations in the present study. Our findings suggest that iodine tincture is mutagenic to human cells.

Citing Articles

Immortalization of normal human gingival keratinocytes and cytological and cytogenetic characterization of the cells.

Kubo C, W Tsutsui T, Tamura Y, Kumakura S, Tsutsui T Odontology. 2009; 97(1):18-31.

PMID: 19184294 DOI: 10.1007/s10266-008-0089-9.

References

Tsutsui T, Hayashi N, Maizumi H, Huff J, Barrett J . Benzene-, catechol-, hydroquinone- and phenol-induced cell transformation, gene mutations, chromosome aberrations, aneuploidy, sister chromatid exchanges and unscheduled DNA synthesis in Syrian hamster embryo cells. Mutat Res. 1997; 373(1):113-23. DOI: 10.1016/s0027-5107(96)00196-0. View

W Tsutsui T, Inaba T, Fisher L, Robey P, Tsutsui T . In vitro chromosome aberration tests using human dental pulp cells to detect the carcinogenic potential of chemical agents. Odontology. 2006; 94(1):44-50. DOI: 10.1007/s10266-006-0065-1. View

HAMAGUCHI F, Tsutsui T . Assessment of genotoxicity of dental antiseptics: ability of phenol, guaiacol, p-phenolsulfonic acid, sodium hypochlorite, p-chlorophenol, m-cresol or formaldehyde to induce unscheduled DNA synthesis in cultured Syrian hamster embryo cells. Jpn J Pharmacol. 2000; 83(3):273-6. DOI: 10.1254/jjp.83.273. View

Sakagami Y, Yamazaki H, Ogasawara N, Yokoyama H, Ose Y, Sato T . The evaluation of genotoxic activities of disinfectants and their metabolites by umu test. Mutat Res. 1988; 209(3-4):155-60. DOI: 10.1016/0165-7992(88)90034-6. View

Tsutsui T, Suzuki N, Maizumi H, Barrett J . Characterization of an unscheduled DNA synthesis assay with Syrian hamster embryo cells. Mutat Res. 1984; 129(1):111-7. DOI: 10.1016/0027-5107(84)90129-5. View

BARTELSTONE H . Radioiodine penetration through intact enamel with uptake by bloodstream and thyroid gland. J Dent Res. 1951; 30(5):728-33. DOI: 10.1177/00220345510300051601. View

Phillips B, Jenkinson P . Is ethanol genotoxic? A review of the published data. Mutagenesis. 2001; 16(2):91-101. DOI: 10.1093/mutage/16.2.91. View

Hikiba H, Watanabe E, Barrett J, Tsutsui T . Ability of fourteen chemical agents used in dental practice to induce chromosome aberrations in Syrian hamster embryo cells. J Pharmacol Sci. 2005; 97(1):146-52. DOI: 10.1254/jphs.fpj04044x. View

Hagiwara M, Watanabe E, Barrett J, Tsutsui T . Assessment of genotoxicity of 14 chemical agents used in dental practice: ability to induce chromosome aberrations in Syrian hamster embryo cells. Mutat Res. 2006; 603(2):111-20. DOI: 10.1016/j.mrgentox.2005.08.011. View

10.

Yamaguchi F, Tsutsui T . Cell-transforming activity of fourteen chemical agents used in dental practice in Syrian hamster embryo cells. J Pharmacol Sci. 2004; 93(4):497-500. DOI: 10.1254/jphs.93.497. View

11.

Whittaker P, Seifried H, San R, Clarke J, Dunkel V . Genotoxicity of iron chelators in L5178Y mouse lymphoma cells. Environ Mol Mutagen. 2002; 38(4):347-56. DOI: 10.1002/em.10033. View

12.

Galloway S, Aardema M, ISHIDATE Jr M, Ivett J, Kirkland D, Morita T . Report from working group on in vitro tests for chromosomal aberrations. Mutat Res. 1994; 312(3):241-61. DOI: 10.1016/0165-1161(94)00012-3. View

13.

Kuroki T, Malaveille C, Drevon C, Piccoli C, MacLeod M, Selkirk J . Critical importance of microsome concentration in mutagenesis assay with V79 Chinese hamster cells. Mutat Res. 1979; 63(2):259-72. DOI: 10.1016/0027-5107(79)90058-7. View

14.

McCann J, Choi E, Yamasaki E, Ames B . Detection of carcinogens as mutagens in the Salmonella/microsome test: assay of 300 chemicals. Proc Natl Acad Sci U S A. 1975; 72(12):5135-9. PMC: 388891. DOI: 10.1073/pnas.72.12.5135. View

15.

Miyachi T, Tsutsui T . Ability of 13 chemical agents used in dental practice to induce sister-chromatid exchanges in Syrian hamster embryo cells. Odontology. 2005; 93(1):24-9. DOI: 10.1007/s10266-005-0055-8. View

16.

BASRUR V, Baker D . Human chromosome breakage in low-calcium cultures. Lancet. 1963; 1(7290):1106-7. DOI: 10.1016/s0140-6736(63)92141-x. View

17.

Fukuda S . [Assessment of the carcinogenic hazard of 6 substances used in dental practices. (1) Morphological transformation, DNA damage and sister chromatid exchanges in cultured Syrian hamster embryo cells induced by carbol camphor, eugenol, thymol, EDTA,...]. Shigaku. 1987; 74(6):1365-84. View

18.

Parshad R, Taylor W, SANFORD K, Camalier R, GANTT R, Tarone R . Fluorescent light-induced chromosome damage in human IMR-90 fibroblasts. Role of hydrogen peroxide and related free radicals. Mutat Res. 1980; 73(1):115-24. DOI: 10.1016/0027-5107(80)90140-2. View

19.

Heindorff K, AURICH O, Michaelis A, Rieger R . Genetic toxicology of ethylenediaminetetraacetic acid (EDTA). Mutat Res. 1983; 115(2):149-73. DOI: 10.1016/0165-1110(83)90001-5. View