Quantitative Evaluation of Sensitivity and Selectivity of Multiplex NanoSPR Biosensor Assays

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2007 Jul 31

PMID 17660314

Citations 13

Authors

Chenxu Yu

Joseph Irudayaraj

Affiliations

Soon will be listed here.

Abstract

A new functionalization procedure was developed to replace cyltrimethylammoniumbromide coating on gold nanorods (GNRs) fabricated through seed-mediated growth with chemically active alkanethiols; antibodies were then attached to the GNRs to yield gold nanorod molecular probes (GNrMPs). The functionalization procedure was shown to minimize nonspecific binding. Multiplex sensing was demonstrated for three targets (goat anti-human IgG, goat anti-rabbit IgG, and goat anti-mouse IgG) through the distinct response of the plasmon spectra of GNrMPs to binding events. Quantification of the plasmonic binding events and estimation of ligand binding kinetics tethered to these nanoscale structures was also demonstrated through a mathematical approach. Evaluation of the experimental and theoretical data yields an affinity constant K(a) = 1.34 x 10(7) M(-1), which was in agreement with the IgG-antiIgG binding affinity reported in the literature. The GNrMP sensors were found to be highly specific and sensitive with the dynamic response in the range between 10(-9) M and 10(-6) M. The limit of detection of GNrMPs was found to be in the low nanomolar range, and is a function of the binding affinity: for a higher probe-target affinity pair, the limit of detection can be expected to reach femto molar levels. This technique can play a key role in developing tunable sensors for sensitive and precise monitoring of biological interactions.

Citing Articles

Multiplex NanoSPR Molecular Biosensor for Blood Cytokine Monitoring.

Mirza A Adv Pharm Bull. 2022; 12(3):419-422.

PMID: 35935058 PMC: 9348532. DOI: 10.34172/apb.2022.046.

Surface Plasmon Resonance Assay for Label-Free and Selective Detection of HIV-1 p24 Protein.

Sarcina L, Mangiatordi G, Torricelli F, Bollella P, Gounani Z, Osterbacka R Biosensors (Basel). 2021; 11(6).

PMID: 34204930 PMC: 8229864. DOI: 10.3390/bios11060180.

Nanosensors based on LSPR are able to serologically differentiate dengue from Zika infections.

Versiani A, Martins E, Andrade L, Cox L, Pereira G, Barbosa-Stancioli E Sci Rep. 2020; 10(1):11302.

PMID: 32647259 PMC: 7347616. DOI: 10.1038/s41598-020-68357-9.

Facile phase transfer of gold nanorods and nanospheres stabilized with block copolymers.

Derikov Y, Shandryuk G, Talroze R, Ezhov A, Kudryavtsev Y Beilstein J Nanotechnol. 2018; 9:616-627.

PMID: 29527437 PMC: 5827792. DOI: 10.3762/bjnano.9.58.

A Sensitive and Stable Surface Plasmon Resonance Sensor Based on Monolayer Protected Silver Film.

Wang G, Wang C, Yang R, Liu W, Sun S Sensors (Basel). 2017; 17(12).

PMID: 29189753 PMC: 5751622. DOI: 10.3390/s17122777.

References

Thevenot D, Toth K, Durst R, Wilson G . Electrochemical biosensors: recommended definitions and classification. Biosens Bioelectron. 2001; 16(1-2):121-31. DOI: 10.1016/s0956-5663(01)00115-4. View

Hall D . Use of optical biosensors for the study of mechanistically concerted surface adsorption processes. Anal Biochem. 2001; 288(2):109-25. DOI: 10.1006/abio.2000.4851. View

Lee S, Perez-Luna V . Dextran-gold nanoparticle hybrid material for biomolecule immobilization and detection. Anal Chem. 2005; 77(22):7204-11. DOI: 10.1021/ac050484n. View

Lee K, El-Sayed M . Dependence of the enhanced optical scattering efficiency relative to that of absorption for gold metal nanorods on aspect ratio, size, end-cap shape, and medium refractive index. J Phys Chem B. 2006; 109(43):20331-8. DOI: 10.1021/jp054385p. View

Caswell K, Wilson J, Bunz U, Murphy C . Preferential end-to-end assembly of gold nanorods by biotin-streptavidin connectors. J Am Chem Soc. 2003; 125(46):13914-5. DOI: 10.1021/ja037969i. View

Haes A, Van Duyne R . A nanoscale optical biosensor: sensitivity and selectivity of an approach based on the localized surface plasmon resonance spectroscopy of triangular silver nanoparticles. J Am Chem Soc. 2002; 124(35):10596-604. DOI: 10.1021/ja020393x. View

Schuck P . Use of surface plasmon resonance to probe the equilibrium and dynamic aspects of interactions between biological macromolecules. Annu Rev Biophys Biomol Struct. 1997; 26:541-66. DOI: 10.1146/annurev.biophys.26.1.541. View

Welschof M, Terness P, Kipriyanov S, Stanescu D, Breitling F, Dorsam H . The antigen-binding domain of a human IgG-anti-F(ab')2 autoantibody. Proc Natl Acad Sci U S A. 1997; 94(5):1902-7. PMC: 20015. DOI: 10.1073/pnas.94.5.1902. View

Voros J . The density and refractive index of adsorbing protein layers. Biophys J. 2004; 87(1):553-61. PMC: 1304376. DOI: 10.1529/biophysj.103.030072. View

10.

Yonzon C, Jeoung E, Zou S, Schatz G, Mrksich M, Van Duyne R . A comparative analysis of localized and propagating surface plasmon resonance sensors: the binding of concanavalin a to a monosaccharide functionalized self-assembled monolayer. J Am Chem Soc. 2004; 126(39):12669-76. DOI: 10.1021/ja047118q. View

11.

Takahashi H, Niidome Y, Niidome T, Kaneko K, Kawasaki H, Yamada S . Modification of gold nanorods using phosphatidylcholine to reduce cytotoxicity. Langmuir. 2005; 22(1):2-5. DOI: 10.1021/la0520029. View

12.

Turner A . Tech.Sight. Biochemistry. Biosensors--sense and sensitivity. Science. 2001; 290(5495):1315-7. DOI: 10.1126/science.290.5495.1315. View

13.

Cao Y, Jin R, Mirkin C . Nanoparticles with Raman spectroscopic fingerprints for DNA and RNA detection. Science. 2002; 297(5586):1536-40. DOI: 10.1126/science.297.5586.1536. View

14.

Lee K, El-Sayed M . Gold and silver nanoparticles in sensing and imaging: sensitivity of plasmon response to size, shape, and metal composition. J Phys Chem B. 2006; 110(39):19220-5. DOI: 10.1021/jp062536y. View

15.

Polla D, Erdman A, Robbins W, Markus D, Rizq R, Nam Y . Microdevices in medicine. Annu Rev Biomed Eng. 2001; 2:551-76. DOI: 10.1146/annurev.bioeng.2.1.551. View

16.

Wang J, Cai X, Rivas G, Shiraishi H, Farias P, Dontha N . DNA electrochemical biosensor for the detection of short DNA sequences related to the human immunodeficiency virus. Anal Chem. 1996; 68(15):2629-34. DOI: 10.1021/ac9602433. View

17.

Nath N, Chilkoti A . Label free colorimetric biosensing using nanoparticles. J Fluoresc. 2004; 14(4):377-89. DOI: 10.1023/b:jofl.0000031819.45448.dc. View

18.

Mascini M, Palchetti I, Marrazza G . DNA electrochemical biosensors. Fresenius J Anal Chem. 2001; 369(1):15-22. DOI: 10.1007/s002160000629. View

19.

Chemla Y, Grossman H, Poon Y, McDermott R, Stevens R, Alper M . Ultrasensitive magnetic biosensor for homogeneous immunoassay. Proc Natl Acad Sci U S A. 2000; 97(26):14268-72. PMC: 18907. DOI: 10.1073/pnas.97.26.14268. View

20.

Kandimalla V, Neeta N, Karanth N, Thakur M, Roshini K, Rani B . Regeneration of ethyl parathion antibodies for repeated use in immunosensor: a study on dissociation of antigens from antibodies. Biosens Bioelectron. 2004; 20(4):903-6. DOI: 10.1016/j.bios.2004.03.027. View