Polymerase-catalyzed Synthesis of DNA from Phosphoramidate Conjugates of Deoxynucleotides and Amino Acids

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2007 Jul 27

PMID 17652326

Citations 10

Authors

O Adelfinskaya

M Terrazas

M Froeyen

P Marliere

K Nauwelaerts

P Herdewijn

Affiliations

Soon will be listed here.

Abstract

Some selected amino acids, in particular L-aspartic acid (L-Asp) and L-histidine (L-His), can function as leaving group during polymerase-catalyzed incorporation of deoxyadenosine monophosphate (dAMP) in DNA. Although L-Asp-dAMP and L-His-dAMP bind, most probably, in a different way in the active site of the enzyme, aspartic acid and histidine can be considered as mimics of the pyrophosphate moiety of deoxyadenosine triphosphate. L-Aspartic acid is more efficient than D-aspartic acid as leaving group. Such P-N conjugates of amino acids and deoxynucleotides provide a novel experimental ground for diversifying nucleic acid metabolism in the field of synthetic biology.

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