Hyperbaric Oxygen Therapy Mediates Increased Nitric Oxide Production Associated with Wound Healing: a Preliminary Study

Overview

Journal Adv Skin Wound Care

Specialty Nursing

Date 2007 Jul 11

PMID 17620739

Citations 26

Authors

Joseph V Boykin Jr

Chris Baylis

Affiliations

Soon will be listed here.

Abstract

Objective: The objective of this preliminary study was to document general somatic and wound nitric oxide (NO) levels during and after hyperbaric oxygen therapy (HBOT).

Design: The study evaluated 6 chronic wound patients that responded favorably to HBOT treatment (20 treatments; 2.0 atmosphere absolute [ATA] x 90 minutes). Successful HBOT was associated with increased wound granulation tissue formation and significantly improved wound closure. Wound fluid and fasting plasma samples were obtained for measurement of nitrate and nitrite (NOx), the stable oxidation products of NO; plasma L-arginine (L-Arg); and asymmetric dimethylarginine (ADMA). NOx measurements were obtained before treatment (baseline), after 10 and 20 treatments, and at 1 and 4 weeks after therapy.

Results: Wound fluid NOx levels tended to increase during treatments, were significantly elevated at 1 and 4 weeks after therapy, and correlated with reductions in wound area. Plasma L-Arg and ADMA were unchanged during and after HBOT.

Conclusion: This preliminary study documents a significant increase in local wound NO levels (by NOx measurements) after successful HBOT and suggests that this mechanism may be an important factor in promoting enhanced wound healing and wound closure associated with this therapy.

Citing Articles

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References

Schaffer M, Tantry U, Efron P, Ahrendt G, Thornton F, Barbul A . Diabetes-impaired healing and reduced wound nitric oxide synthesis: a possible pathophysiologic correlation. Surgery. 1997; 121(5):513-9. DOI: 10.1016/s0039-6060(97)90105-7. View

Schaffer M, Tantry U, Ahrendt G, Wasserkrug H, Barbul A . Acute protein-calorie malnutrition impairs wound healing: a possible role of decreased wound nitric oxide synthesis. J Am Coll Surg. 1997; 184(1):37-43. View

Schaffer M, Tantry U, Van Wesep R, Barbul A . Nitric oxide metabolism in wounds. J Surg Res. 1997; 71(1):25-31. DOI: 10.1006/jsre.1997.5137. View

Anderstam B, Katzarski K, Bergstrom J . Serum levels of NG, NG-dimethyl-L-arginine, a potential endogenous nitric oxide inhibitor in dialysis patients. J Am Soc Nephrol. 1997; 8(9):1437-42. DOI: 10.1681/ASN.V891437. View

Brady T, Chang L, Day B, Crapo J . Extracellular superoxide dismutase is upregulated with inducible nitric oxide synthase after NF-kappa B activation. Am J Physiol. 1997; 273(5):L1002-6. DOI: 10.1152/ajplung.1997.273.5.L1002. View

Baylis C, Vallance P . Measurement of nitrite and nitrate levels in plasma and urine--what does this measure tell us about the activity of the endogenous nitric oxide system?. Curr Opin Nephrol Hypertens. 1998; 7(1):59-62. DOI: 10.1097/00041552-199801000-00010. View

Yamasaki K, Edington H, McClosky C, Tzeng E, Lizonova A, Kovesdi I . Reversal of impaired wound repair in iNOS-deficient mice by topical adenoviral-mediated iNOS gene transfer. J Clin Invest. 1998; 101(5):967-71. PMC: 508647. DOI: 10.1172/JCI2067. View

Um S, Suzuki S, Toyokuni S, Kim B, Tanaka T, Hiai H . Involvement of nitric oxide in survival of random pattern skin flap. Plast Reconstr Surg. 1998; 101(3):785-92. DOI: 10.1097/00006534-199803000-00030. View

Bonomo S, DAVIDSON J, Yu Y, Xia Y, Lin X, Mustoe T . Hyperbaric oxygen as a signal transducer: upregulation of platelet derived growth factor-beta receptor in the presence of HBO2 and PDGF. Undersea Hyperb Med. 1999; 25(4):211-6. View

10.

Schaffer M, Tantry U, Thornton F, Barbul A . Inhibition of nitric oxide synthesis in wounds: pharmacology and effect on accumulation of collagen in wounds in mice. Eur J Surg. 1999; 165(3):262-7. DOI: 10.1080/110241599750007153. View

11.

Schmidt R, Yokota S, Tracy T, Sorkin M, Baylis C . Nitric oxide production is low in end-stage renal disease patients on peritoneal dialysis. Am J Physiol. 1999; 276(5):F794-7. PMC: 2793680. DOI: 10.1152/ajprenal.1999.276.5.F794. View

12.

Hristov M, Weber C . Endothelial progenitor cells: characterization, pathophysiology, and possible clinical relevance. J Cell Mol Med. 2004; 8(4):498-508. PMC: 6740289. DOI: 10.1111/j.1582-4934.2004.tb00474.x. View

13.

Thom S, Bhopale V, Velazquez O, Goldstein L, Thom L, Buerk D . Stem cell mobilization by hyperbaric oxygen. Am J Physiol Heart Circ Physiol. 2005; 290(4):H1378-86. DOI: 10.1152/ajpheart.00888.2005. View

14.

Baylis C . Arginine, arginine analogs and nitric oxide production in chronic kidney disease. Nat Clin Pract Nephrol. 2006; 2(4):209-20. PMC: 2756810. DOI: 10.1038/ncpneph0143. View

15.

Shi H, Efron D, Most D, Tantry U, Barbul A . Supplemental dietary arginine enhances wound healing in normal but not inducible nitric oxide synthase knockout mice. Surgery. 2000; 128(2):374-8. DOI: 10.1067/msy.2000.107372. View

16.

Boykin Jr J . The nitric oxide connection: hyperbaric oxygen therapy, becaplermin, and diabetic ulcer management. Adv Skin Wound Care. 2000; 13(4 Pt 1):169-74. View

17.

Fukumura D, Gohongi T, Kadambi A, Izumi Y, Ang J, Yun C . Predominant role of endothelial nitric oxide synthase in vascular endothelial growth factor-induced angiogenesis and vascular permeability. Proc Natl Acad Sci U S A. 2001; 98(5):2604-9. PMC: 30185. DOI: 10.1073/pnas.041359198. View

18.

Pollock J, Webb W, Callaway D, Sathyanarayana , OBrien W, Howdieshell T . Nitric oxide synthase isoform expression in a porcine model of granulation tissue formation. Surgery. 2001; 129(3):341-50. DOI: 10.1067/msy.2001.111700. View

19.

Lee R, Efron D, Tantry U, Barbul A . Nitric oxide in the healing wound: a time-course study. J Surg Res. 2001; 101(1):104-8. DOI: 10.1006/jsre.2001.6261. View

20.

Boykin Jr J . Re: Oxygen in wound healing: more than a nutrient. Wound Repair Regen. 2002; 9(5):391-2. DOI: 10.1046/j.1524-475x.2001.00391.x. View