MiR172 Regulates Stem Cell Fate and Defines the Inner Boundary of APETALA3 and PISTILLATA Expression Domain in Arabidopsis Floral Meristems

Overview

Journal Plant J

Specialties Biology
Cell Biology
Molecular Biology

Date 2007 Jun 19

PMID 17573799

Citations 89

Authors

Li Zhao

Yunju Kim

Theresa T Dinh

Xuemei Chen

Affiliations

Soon will be listed here.

Abstract

In Arabidopsis, two floral homeotic genes APETALA2 (AP2) and AGAMOUS (AG) specify the identities of perianth and reproductive organs, respectively, in flower development. The two genes act antagonistically to restrict each other to their proper domains of action within the floral meristem. In addition to AG, which antagonizes AP2, miR172, a microRNA, serves as a negative regulator of AP2. In this study, we showed that AG and miR172 have distinct functions in flower development and that they largely act independently in the negative regulation of AP2. We uncovered functions of miR172-mediated repression of AP2 in the regulation of floral stem cells and in the delineation of the expression domain of another class of floral homeotic genes. Given the antiquity of miR172 in land plants, our findings have implications for the recruitment of a microRNA in the building of a flower in evolution.

Citing Articles

Identification and characterization of two APETALA2 homolog genes in lotus (Nelumbo nucifera) involved in sepal and petal development.

Xuelian L, Yu Q, Dingding C, Jiao D, Songbiao C, Pingfang Y BMC Plant Biol. 2024; 24(1):1186.

PMID: 39695956 PMC: 11654349. DOI: 10.1186/s12870-024-05923-7.

Molecular Manipulation of the miR160/ Expression Module Impacts Root Development in .

Zimmerman K, Pegler J, Oultram J, Collings D, Wang M, Grof C Genes (Basel). 2024; 15(8).

PMID: 39202402 PMC: 11353855. DOI: 10.3390/genes15081042.

Transcription factors HB21/40/53 trigger inflorescence arrest through abscisic acid accumulation at the end of flowering.

Sanchez-Gerschon V, Martinez-Fernandez I, Gonzalez-Bermudez M, de la Hoz-Rodriguez S, Gonzalez F, Lozano-Juste J Plant Physiol. 2024; 195(4):2743-2756.

PMID: 38669447 PMC: 11288733. DOI: 10.1093/plphys/kiae234.

Mutations overlying the miR172 target site of TOE-type genes are prime candidate variants for the double-flower trait in mei.

Gattolin S, Calastri E, Tassone M, Cirilli M Sci Rep. 2024; 14(1):7300.

PMID: 38538684 PMC: 10973477. DOI: 10.1038/s41598-024-57589-8.

Genome-wide identification and characterization of protein phosphatase 2C (PP2C) gene family in sunflower (Helianthus annuus L.) and their expression profiles in response to multiple abiotic stresses.

Akter N, Islam M, Rahman M, Zohra F, Rahman S, Manirujjaman M PLoS One. 2024; 19(3):e0298543.

PMID: 38507444 PMC: 10954154. DOI: 10.1371/journal.pone.0298543.

References

Schoof H, Lenhard M, Haecker A, Mayer K, Jurgens G, Laux T . The stem cell population of Arabidopsis shoot meristems in maintained by a regulatory loop between the CLAVATA and WUSCHEL genes. Cell. 2000; 100(6):635-44. DOI: 10.1016/s0092-8674(00)80700-x. View

Brand U, Hobe M, Simon R . Functional domains in plant shoot meristems. Bioessays. 2001; 23(2):134-41. DOI: 10.1002/1521-1878(200102)23:2<134::AID-BIES1020>3.0.CO;2-3. View

Clark S . Cell signalling at the shoot meristem. Nat Rev Mol Cell Biol. 2001; 2(4):276-84. DOI: 10.1038/35067079. View

Lohmann J, Hong R, Hobe M, Busch M, Parcy F, Simon R . A molecular link between stem cell regulation and floral patterning in Arabidopsis. Cell. 2001; 105(6):793-803. DOI: 10.1016/s0092-8674(01)00384-1. View

Lenhard M, Bohnert A, Jurgens G, Laux T . Termination of stem cell maintenance in Arabidopsis floral meristems by interactions between WUSCHEL and AGAMOUS. Cell. 2001; 105(6):805-14. DOI: 10.1016/s0092-8674(01)00390-7. View

Chen X, Liu J, Cheng Y, Jia D . HEN1 functions pleiotropically in Arabidopsis development and acts in C function in the flower. Development. 2002; 129(5):1085-94. PMC: 5137379. DOI: 10.1242/dev.129.5.1085. View

Park W, Li J, Song R, Messing J, Chen X . CARPEL FACTORY, a Dicer homolog, and HEN1, a novel protein, act in microRNA metabolism in Arabidopsis thaliana. Curr Biol. 2002; 12(17):1484-95. PMC: 5137372. DOI: 10.1016/s0960-9822(02)01017-5. View

Sharma V, Carles C, Fletcher J . Maintenance of stem cell populations in plants. Proc Natl Acad Sci U S A. 2003; 100 Suppl 1:11823-9. PMC: 304093. DOI: 10.1073/pnas.1834206100. View

Aukerman M, Sakai H . Regulation of flowering time and floral organ identity by a MicroRNA and its APETALA2-like target genes. Plant Cell. 2003; 15(11):2730-41. PMC: 280575. DOI: 10.1105/tpc.016238. View

10.

Chen X . A microRNA as a translational repressor of APETALA2 in Arabidopsis flower development. Science. 2003; 303(5666):2022-5. PMC: 5127708. DOI: 10.1126/science.1088060. View

11.

Jack T . Molecular and genetic mechanisms of floral control. Plant Cell. 2004; 16 Suppl:S1-17. PMC: 2643400. DOI: 10.1105/tpc.017038. View

12.

Yanofsky M, Ma H, Bowman J, Drews G, Feldmann K, Meyerowitz E . The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors. Nature. 1990; 346(6279):35-9. DOI: 10.1038/346035a0. View

13.

Drews G, Bowman J, Meyerowitz E . Negative regulation of the Arabidopsis homeotic gene AGAMOUS by the APETALA2 product. Cell. 1991; 65(6):991-1002. DOI: 10.1016/0092-8674(91)90551-9. View

14.

Bowman J, Smyth D, Meyerowitz E . Genetic interactions among floral homeotic genes of Arabidopsis. Development. 1991; 112(1):1-20. DOI: 10.1242/dev.112.1.1. View

15.

Jack T, Brockman L, Meyerowitz E . The homeotic gene APETALA3 of Arabidopsis thaliana encodes a MADS box and is expressed in petals and stamens. Cell. 1992; 68(4):683-97. DOI: 10.1016/0092-8674(92)90144-2. View

16.

Bowman J, Sakai H, Jack T, Weigel D, Mayer U, Meyerowitz E . SUPERMAN, a regulator of floral homeotic genes in Arabidopsis. Development. 1992; 114(3):599-615. DOI: 10.1242/dev.114.3.599. View

17.

Bowman J, Drews G, Meyerowitz E . Expression of the Arabidopsis floral homeotic gene AGAMOUS is restricted to specific cell types late in flower development. Plant Cell. 1991; 3(8):749-58. PMC: 160042. DOI: 10.1105/tpc.3.8.749. View

18.

Bowman J, Smyth D, Meyerowitz E . Genes directing flower development in Arabidopsis. Plant Cell. 1989; 1(1):37-52. PMC: 159735. DOI: 10.1105/tpc.1.1.37. View

19.

Mizukami Y, Ma H . Ectopic expression of the floral homeotic gene AGAMOUS in transgenic Arabidopsis plants alters floral organ identity. Cell. 1992; 71(1):119-31. DOI: 10.1016/0092-8674(92)90271-d. View

20.

Mandel M, Savidge B, Yanofsky M . Molecular characterization of the Arabidopsis floral homeotic gene APETALA1. Nature. 1992; 360(6401):273-7. DOI: 10.1038/360273a0. View