Sinorhizobium Meliloti Fur-like (Mur) Protein Binds a Fur Box-like Sequence Present in the MntA Promoter in a Manganese-responsive Manner

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2007 Jun 15

PMID 17557847

Citations 20

Authors

Raul Platero

Victor de Lorenzo

Beatriz Garat

Elena Fabiano

Affiliations

Soon will be listed here.

Abstract

In Sinorhizobium meliloti, the Mur(Sm) protein, a homologue of the ferric uptake regulator (Fur), mediates manganese-dependent regulation of the MntABCD manganese uptake system. In this study, we analyzed Mur(Sm) binding to the promoter region of the S. meliloti mntA gene. We demonstrated that Mur(Sm) protein binds with high affinity to the promoter region of mntA gene in a manganese-responsive manner. Moreover, the results presented here indicate that two monomers, or one dimer, of Mur(Sm) binds the DNA. The binding region was identified by DNase I footprinting analysis and covers a region of about 30 bp long that contains a palindromic sequence. The Mur(Sm) binding site, present in the mntA promoter region, is similar to a Fur box; however, manganese-activated Mur(Sm) binds a canonical Fur box with very low affinity. Furthermore, the data obtained indicate that Mur(Sm) responds to physiological concentrations of manganese.

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