Fur Regulon of Salmonella Typhimurium: Identification of New Iron-regulated Genes

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1995 Aug 1

PMID 7642488

Citations 66

Authors

R M Tsolis

A J Baumler

I Stojiljkovic

F Heffron

Affiliations

Soon will be listed here.

Abstract

In order to identify genes belonging to the Fur regulon of Salmonella typhimurium, a bank of 10,000 independent S. typhimurium MudJ insertion mutants was screened for lacZ fusions regulated by the iron response regulator Fur. In parallel, a plasmid gene bank of S. typhimurium consisting of 10,000 independent clones was screened for Fur-regulated promoters or iron binding proteins by the Fur titration assay (FURTA). Fur-regulated MudJ insertions and Fur-regulated promoters were mapped. In addition, iron-regulated promoter activities of transcriptional fusions from MudJ insertions and FURTA-positive clones were quantified. The nucleotide sequences of 11 FURTA-positive plasmids and of short fragments of DNA flanking three MudJ insertions were determined. By these methods we identified 14 Fur-regulated genes of S. typhimurium. For 11 of these genes, Fur-regulated homologs have been described in Escherichia coli or Yersinia enterocolitica, including fhuA,fhuB,fepA,fes,fepD,p43,entB,fur ,foxA,hemP, and fhuE. In addition, we identified three genes with homologs in other bacteria which have not previously been shown to be Fur regulated.

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References

de Lorenzo V, Herrero M, Giovannini F, NEILANDS J . Fur (ferric uptake regulation) protein and CAP (catabolite-activator protein) modulate transcription of fur gene in Escherichia coli. Eur J Biochem. 1988; 173(3):537-46. DOI: 10.1111/j.1432-1033.1988.tb14032.x. View

Hantke K . Selection procedure for deregulated iron transport mutants (fur) in Escherichia coli K 12: fur not only affects iron metabolism. Mol Gen Genet. 1987; 210(1):135-9. DOI: 10.1007/BF00337769. View

Nakamura H, Murakami H, Yamato I, Anraku Y . Nucleotide sequence of the cybB gene encoding cytochrome b561 in Escherichia coli K12. Mol Gen Genet. 1988; 212(1):1-5. DOI: 10.1007/BF00322437. View

Hughes K, Roth J . Transitory cis complementation: a method for providing transposition functions to defective transposons. Genetics. 1988; 119(1):9-12. PMC: 1203349. DOI: 10.1093/genetics/119.1.9. View

Nahlik M, Brickman T, Ozenberger B, McIntosh M . Nucleotide sequence and transcriptional organization of the Escherichia coli enterobactin biosynthesis cistrons entB and entA. J Bacteriol. 1989; 171(2):784-90. PMC: 209665. DOI: 10.1128/jb.171.2.784-790.1989. View

Liu J, Duncan K, WALSH C . Nucleotide sequence of a cluster of Escherichia coli enterobactin biosynthesis genes: identification of entA and purification of its product 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase. J Bacteriol. 1989; 171(2):791-8. PMC: 209666. DOI: 10.1128/jb.171.2.791-798.1989. View

Staudenmaier H, Van Hove B, Yaraghi Z, Braun V . Nucleotide sequences of the fecBCDE genes and locations of the proteins suggest a periplasmic-binding-protein-dependent transport mechanism for iron(III) dicitrate in Escherichia coli. J Bacteriol. 1989; 171(5):2626-33. PMC: 209944. DOI: 10.1128/jb.171.5.2626-2633.1989. View

Kraft R, Tardiff J, Krauter K, Leinwand L . Using mini-prep plasmid DNA for sequencing double stranded templates with Sequenase. Biotechniques. 1988; 6(6):544-6, 549. View

Hantke K . Dihydroxybenzoylserine--a siderophore for E. coli. FEMS Microbiol Lett. 1990; 55(1-2):5-8. DOI: 10.1016/0378-1097(90)90158-m. View

10.

Sauer M, Hantke K, Braun V . Sequence of the fhuE outer-membrane receptor gene of Escherichia coli K12 and properties of mutants. Mol Microbiol. 1990; 4(3):427-37. DOI: 10.1111/j.1365-2958.1990.tb00609.x. View

11.

de Lorenzo V, Herrero M, Jakubzik U, Timmis K . Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. J Bacteriol. 1990; 172(11):6568-72. PMC: 526846. DOI: 10.1128/jb.172.11.6568-6572.1990. View

12.

Altschul S, Gish W, Miller W, Myers E, Lipman D . Basic local alignment search tool. J Mol Biol. 1990; 215(3):403-10. DOI: 10.1016/S0022-2836(05)80360-2. View

13.

Hannavy K, BARR G, Dorman C, Adamson J, Mazengera L, Gallagher M . TonB protein of Salmonella typhimurium. A model for signal transduction between membranes. J Mol Biol. 1990; 216(4):897-910. DOI: 10.1016/S0022-2836(99)80009-6. View

14.

Coy M, NEILANDS J . Structural dynamics and functional domains of the fur protein. Biochemistry. 1991; 30(33):8201-10. DOI: 10.1021/bi00247a016. View

15.

Chenault S, Earhart C . Organization of genes encoding membrane proteins of the Escherichia coli ferrienterobactin permease. Mol Microbiol. 1991; 5(6):1405-13. DOI: 10.1111/j.1365-2958.1991.tb00787.x. View

16.

Shea C, McIntosh M . Nucleotide sequence and genetic organization of the ferric enterobactin transport system: homology to other periplasmic binding protein-dependent systems in Escherichia coli. Mol Microbiol. 1991; 5(6):1415-28. DOI: 10.1111/j.1365-2958.1991.tb00788.x. View

17.

Wong K, McClelland M . A BlnI restriction map of the Salmonella typhimurium LT2 genome. J Bacteriol. 1992; 174(5):1656-61. PMC: 206563. DOI: 10.1128/jb.174.5.1656-1661.1992. View

18.

Liu S, Sanderson K . A physical map of the Salmonella typhimurium LT2 genome made by using XbaI analysis. J Bacteriol. 1992; 174(5):1662-72. PMC: 206564. DOI: 10.1128/jb.174.5.1662-1672.1992. View

19.

Prodromou C, Artymiuk P, Guest J . The aconitase of Escherichia coli. Nucleotide sequence of the aconitase gene and amino acid sequence similarity with mitochondrial aconitases, the iron-responsive-element-binding protein and isopropylmalate isomerases. Eur J Biochem. 1992; 204(2):599-609. DOI: 10.1111/j.1432-1033.1992.tb16673.x. View

20.

FOSTER J, Hall H . Effect of Salmonella typhimurium ferric uptake regulator (fur) mutations on iron- and pH-regulated protein synthesis. J Bacteriol. 1992; 174(13):4317-23. PMC: 206215. DOI: 10.1128/jb.174.13.4317-4323.1992. View