A High-throughput Assay for O-GlcNAc Transferase Detects Primary Sequence Preferences in Peptide Substrates

Overview

Journal Bioorg Med Chem Lett

Specialty Biochemistry

Date 2007 May 29

PMID 17531489

Citations 15

Authors

Tanya M Leavy

Carolyn R Bertozzi

Affiliations

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Abstract

O-GlcNAc transferase (OGT) catalyzes the addition of N-acetylglucosamine (O-GlcNAc) onto a diverse array of intracellular proteins. Although hundreds of proteins are known to be modified by O-GlcNAc, a strict amino acid consensus sequence for OGT has not been identified. In this study, we describe the development of a high-throughput assay for OGT and use it to profile the specificity of the enzyme among a panel of peptide substrates.

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