Structure of GrlR and the Implication of Its EDED Motif in Mediating the Regulation of Type III Secretion System in EHEC

Overview

Journal PLoS Pathog

Specialty Microbiology

Date 2007 May 22

PMID 17511515

Citations 20

Authors

Chacko Jobichen

Mo Li

Gal Yerushalmi

Yih Wan Tan

Yu-Keung Mok

Ilan Rosenshine

Ka Yin Leung

J Sivaraman

Affiliations

Soon will be listed here.

Abstract

Enterohemorrhagic Escherichia coli (EHEC) is a common cause of severe hemorrhagic colitis. EHEC's virulence is dependent upon a type III secretion system (TTSS) encoded by 41 genes. These genes are organized in several operons clustered in the locus of enterocyte effacement. Most of the locus of enterocyte effacement genes, including grlA and grlR, are positively regulated by Ler, and Ler expression is positively and negatively modulated by GrlA and GrlR, respectively. However, the molecular basis for the GrlA and GrlR activity is still elusive. We have determined the crystal structure of GrlR at 1.9 A resolution. It consists of a typical beta-barrel fold with eight beta-strands containing an internal hydrophobic cavity and a plug-like loop on one side of the barrel. Strong hydrophobic interactions between the two beta-barrels maintain the dimeric architecture of GrlR. Furthermore, a unique surface-exposed EDED (Glu-Asp-Glu-Asp) motif is identified to be critical for GrlA-GrlR interaction and for the repressive activity of GrlR. This study contributes a novel molecular insight into the mechanism of GrlR function.

Citing Articles

Phenotypic diversity of type III secretion system activity in enteropathogenic clinical isolates.

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GrlR, a negative regulator in enteropathogenic , also represses the expression of LEE virulence genes independently of its interaction with its cognate partner GrlA.

Lara-Ochoa C, Huerta-Saquero A, Medrano-Lopez A, Deng W, Finlay B, Martinez-Laguna Y Front Microbiol. 2023; 14:1063368.

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The two sRNAs OmrA and OmrB indirectly repress transcription from the LEE1 promoter of enteropathogenic Escherichia coli.

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The transcription factor GrlA is regulated by subcellular compartmentalization and activated in response to mechanical stimuli.

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The Small Regulatory RNA Spot42 Inhibits Indole Biosynthesis to Negatively Regulate the Locus of Enterocyte Effacement of Enteropathogenic Escherichia coli.

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