Dynamics of Expression and Maturation of the Type III Secretion System of Enteropathogenic Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2014 May 20

PMID 24837293

Citations 15

Authors

Gal Yerushalmi

Yael Litvak

Lihi Gur-Arie

Ilan Rosenshine

Affiliations

Soon will be listed here.

Abstract

Enteropathogenic Escherichia coli (EPEC) is a major cause of food poisoning, leading to significant morbidity and mortality. EPEC virulence is dependent on a type III secretion system (T3SS), a molecular syringe employed by EPEC to inject effector proteins into host cells. The injected effector proteins subvert host cellular functions to the benefit of the infecting bacteria. The T3SS and related genes reside in several operons clustered in the locus of enterocyte effacement (LEE). We carried out simultaneous analysis of the expression dynamics of all the LEE promoters and the rate of maturation of the T3SS. The results showed that expression of the LEE1 operon is activated immediately upon shifting the culture to inducing conditions, while expression of other LEE promoters is activated only ∼70 min postinduction. Parallel analysis showed that the T3SS becomes functional around 100 min postinduction. The T3SS core proteins EscS, EscT, EscU, and EscR are predicted to be involved in the first step of T3SS assembly and are therefore included among the LEE1 genes. However, interfering with the temporal regulation of EscS, EscT, EscU, and EscR expression has only a marginal effect on the rate of the T3SS assembly. This study provides a comprehensive description of the transcription dynamics of all the LEE genes and correlates it to that of T3SS biogenesis.

Citing Articles

Phenotypic diversity of type III secretion system activity in enteropathogenic clinical isolates.

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High variation across hybrid isolates identified in metabolism-related biological pathways co-expressed with virulent genes.

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GrlR, a negative regulator in enteropathogenic , also represses the expression of LEE virulence genes independently of its interaction with its cognate partner GrlA.

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The impact of Hfq-mediated sRNA-mRNA interactome on the virulence of enteropathogenic .

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CesL Regulates Type III Secretion Substrate Specificity of the Enteropathogenic Injectisome.

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