B Cell Tolerance in Mice Transgenic for Anti-CD8 Immunoglobulin Mu Chain

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1991 Dec 1

PMID 1744576

Citations 9

Authors

F Brombacher

G Kohler

H Eibel

Affiliations

Soon will be listed here.

Abstract

To analyze in vivo the induction of B cell tolerance against a T cell surface antigen, we generated transgenic mice expressing an anti-CD8.2 mu heavy chain gene. We show that self-specific B cells are efficiently tolerized if they express the membrane-bound form of the transgenic mu chain on their surface but that they can escape tolerization if they express only the secreted form. In the latter, we find an enhanced expression of anti-CD8.2 antibodies after polyclonal B cell activation. As a result, transgenic anti-CD8.2 antibodies bind to the CD8+ T cells but they did not induce their elimination. Furthermore, we observed the preferential expression of a limited subset of endogenous light chains with the transgenic mu chain. This suggests a positive or negative selection for particular heavy and light chain combinations in B lymphocytes.

Citing Articles

Innate B-1 B Cells Are Not Enriched in Red Blood Cell Autoimmune Mice: Importance of B Cell Receptor Transgenic Selection.

Richards A, Howie H, Kapp L, Hendrickson J, Zimring J, Hudson K Front Immunol. 2017; 8:1366.

PMID: 29163471 PMC: 5675845. DOI: 10.3389/fimmu.2017.01366.

B cell biology: an overview.

Eibel H, Kraus H, Sic H, Kienzler A, Rizzi M Curr Allergy Asthma Rep. 2014; 14(5):434.

PMID: 24633618 DOI: 10.1007/s11882-014-0434-8.

Receptor editing in self-reactive bone marrow B cells. The Journal of Experimental Medicine. 1993. 177: 1009-1020.

Tiegs S, Russell D, Nemazee D J Immunol. 2011; 186(3):1313-24.

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The transcription factor early growth response 1 (Egr-1) advances differentiation of pre-B and immature B cells.

Dinkel A, Warnatz K, Ledermann B, Rolink A, Zipfel P, Burki K J Exp Med. 1998; 188(12):2215-24.

PMID: 9858508 PMC: 2212439. DOI: 10.1084/jem.188.12.2215.

Characterization of SV40T antigen immortalized human synovial fibroblasts: maintained expression patterns of EGR-1, HLA-DR and some surface receptors.

Haas C, Aicher W, Dinkel A, Peter H, Eibel H Rheumatol Int. 1997; 16(6):241-7.

PMID: 9106935 DOI: 10.1007/BF01375656.

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