Molecular Architecture and Functional Model of the Complete Yeast ESCRT-I Heterotetramer

Overview

Journal Cell

Publisher Cell Press

Specialty Cell Biology

Date 2007 Apr 20

PMID 17442384

Citations 98

Authors

Michael S Kostelansky

Cayetana Schluter

Yuen Yi C Tam

Sangho Lee

Rodolfo Ghirlando

Bridgette Beach

Elizabeth Conibear

James H Hurley

Affiliations

Soon will be listed here.

Abstract

The endosomal sorting complex required for transport-I (ESCRT-I) complex, which is conserved from yeast to humans, directs the lysosomal degradation of ubiquitinated transmembrane proteins and the budding of the HIV virus. Yeast ESCRT-I contains four subunits, Vps23, Vps28, Vps37, and Mvb12. The crystal structure of the heterotetrameric ESCRT-I complex reveals a highly asymmetric complex of 1:1:1:1 subunit stoichiometry. The core complex is nearly 18 nm long and consists of a headpiece attached to a 13 nm stalk. The stalk is important for cargo sorting by ESCRT-I and is proposed to serve as a spacer regulating the correct disposition of cargo and other ESCRT components. Hydrodynamic constraints and crystallographic structures were used to generate a model of intact ESCRT-I in solution. The results show how ESCRT-I uses a combination of a rigid stalk and flexible tethers to interact with lipids, cargo, and other ESCRT complexes over a span of approximately 25 nm.

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