A Signal from Inside the Peroxisome Initiates Its Division by Promoting the Remodeling of the Peroxisomal Membrane

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2007 Apr 18

PMID 17438077

Citations 34

Authors

Tong Guo

Christopher Gregg

Tatiana Boukh-Viner

Pavlo Kyryakov

Alexander Goldberg

Simon Bourque

Farhana Banu

Sandra Haile

Svetlana Milijevic

Karen Hung Yeung San

Jonathan Solomon

Vivianne Wong

Vladimir I Titorenko

Affiliations

Soon will be listed here.

Abstract

We define the dynamics of spatial and temporal reorganization of the team of proteins and lipids serving peroxisome division. The peroxisome becomes competent for division only after it acquires the complete set of matrix proteins involved in lipid metabolism. Overloading the peroxisome with matrix proteins promotes the relocation of acyl-CoA oxidase (Aox), an enzyme of fatty acid beta-oxidation, from the matrix to the membrane. The binding of Aox to Pex16p, a membrane-associated peroxin required for peroxisome biogenesis, initiates the biosynthesis of phosphatidic acid and diacylglycerol (DAG) in the membrane. The formation of these two lipids and the subsequent transbilayer movement of DAG initiate the assembly of a complex between the peroxins Pex10p and Pex19p, the dynamin-like GTPase Vps1p, and several actin cytoskeletal proteins on the peroxisomal surface. This protein team promotes membrane fission, thereby executing the terminal step of peroxisome division.

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