Rev Proteins of Human and Simian Immunodeficiency Virus Enhance RNA Encapsidation

Overview

Journal PLoS Pathog

Specialty Microbiology

Date 2007 Apr 17

PMID 17432934

Citations 58

Authors

Sabine Brandt

Maik Blissenbach

Bastian Grewe

Rebecca Konietzny

Thomas Grunwald

Klaus Uberla

Affiliations

Soon will be listed here.

Abstract

The main function attributed to the Rev proteins of immunodeficiency viruses is the shuttling of viral RNAs containing the Rev responsive element (RRE) via the CRM-1 export pathway from the nucleus to the cytoplasm. This restricts expression of structural proteins to the late phase of the lentiviral replication cycle. Using Rev-independent gag-pol expression plasmids of HIV-1 and simian immunodeficiency virus and lentiviral vector constructs, we have observed that HIV-1 and simian immunodeficiency virus Rev enhanced RNA encapsidation 20- to 70-fold, correlating well with the effect of Rev on vector titers. In contrast, cytoplasmic vector RNA levels were only marginally affected by Rev. Binding of Rev to the RRE or to a heterologous RNA element was required for Rev-mediated enhancement of RNA encapsidation. In addition to specific interactions of nucleocapsid with the packaging signal at the 5' end of the genome, the Rev/RRE system provides a second mechanism contributing to preferential encapsidation of genomic lentiviral RNA.

Citing Articles

Suppression of viral rebound by a Rev-dependent lentiviral particle in SIV-infected rhesus macaques.

Hetrick B, Siddiqui S, Spear M, Guo J, Liang H, Fu Y Gene Ther. 2024; 32(1):16-24.

PMID: 39025983 PMC: 11785524. DOI: 10.1038/s41434-024-00467-9.

HIV-1 RNA genome packaging: it's G-rated.

Duchon A, Hu W mBio. 2024; 15(4):e0086123.

PMID: 38411060 PMC: 11005445. DOI: 10.1128/mbio.00861-23.

Expression, purification, and functional characterization of soluble recombinant full-length simian immunodeficiency virus (SIV) Pr55.

Pillai V, Ali L, Prabhu S, Krishnan A, Tariq S, Mustafa F Heliyon. 2023; 9(1):e12892.

PMID: 36685375 PMC: 9853374. DOI: 10.1016/j.heliyon.2023.e12892.

Binding stoichiometry and structural model of the HIV-1 Rev/importin β complex.

Spittler D, Indorato R, Boeri Erba E, Delaforge E, Signor L, Harris S Life Sci Alliance. 2022; 5(10).

PMID: 35995566 PMC: 9396022. DOI: 10.26508/lsa.202201431.

RNA Structures and Their Role in Selective Genome Packaging.

Ye L, Ambi U, Olguin-Nava M, Gribling-Burrer A, Ahmad S, Bohn P Viruses. 2021; 13(9).

PMID: 34578369 PMC: 8472981. DOI: 10.3390/v13091788.

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