The MicroRNA MiR-124 Antagonizes the Anti-neural REST/SCP1 Pathway During Embryonic CNS Development

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2007 Apr 4

PMID 17403776

Citations 341

Authors

Jaya Visvanathan

Seunghee Lee

Bora Lee

Jae W Lee

Soo-Kyung Lee

Affiliations

Soon will be listed here.

Abstract

Neuronal gene expression is tightly regulated in developing CNS. Here, we demonstrate the anti-neural function of phosphatase SCP1 (small C-terminal domain phosphatase 1) during development. We further show that the neuron-enriched microRNA miR-124 directly targets SCP1-3' untranslated region (UTR) to suppress SCP1 expression. In developing spinal cord, expression of miR-124 and SCP1 is complementary, and miR-124 antagonism phenocopies SCP1 overexpression and vice versa. In P19 cells, miR-124 suppresses SCP1 expression and induces neurogenesis, and SCP1 counteracts this proneural activity of miR-124. Our results suggest that, during CNS development, timely down-regulation of SCP1 is critical for inducing neurogenesis, and miR-124 contributes to this process at least in part by down-regulating SCP1 expression.

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