E and EAC Rosetting Lymphocytes in Patients with Carcinoma of Bronchus I. Some Parameters of the Test and of Its Prognostic Significance

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1975 Apr 1

PMID 173486

Citations 11

Authors

H M Anthony

J A Kirk

K E Madsen

M K MASON

G H Templeman

Affiliations

Soon will be listed here.

Abstract

Using peripheral blood lymphocytes separated by a Ficoll method and suspended in saline, means of 77-1% (s.d. 5-2) E rosettes (T lymphocytes) and 20-1% (s.d. 6-7) EAC rosettes (B lymphocytes) have been obtained with normal healthy donors. Poorer E-rosette formation resulted from higher centrifugation speeds during the washing of lymphocytes or erythrocytes, insufficient chilling, or rough handling. The presence of 5% albumin in the final mixture stabilized the rosettes and brought a constant subpopulation of B lymphocytes into rosetting. In patients with bronchial carcinoma who, at the time of diagnosis, had E-rosette percentages below 1 s.d. of the mean for normal donors, the length of survival was significantly shorter than in those with normal or high values. The same was true for those in whom null cells were detected. In each case the correlation effect was mainly found in the group of patients with squamous carcinoma.

Citing Articles

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Changes of peripheral T lymphocyte subsets following treatment in patients with bronchogenic carcinoma.

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The effects of azathioprine and levamisole on rosette-forming cells of healthy subjects and cancer patients.

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Relative and absolute numbers of E- and EAC-rosette-forming cells in lymphoid organs.

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