An MRNA Sequence Derived from the Yeast EST3 Gene Stimulates Programmed +1 Translational Frameshifting

Overview

Journal RNA

Specialty Molecular Biology

Date 2007 Mar 3

PMID 17329356

Citations 10

Authors

Dwayne Taliaferro

Philip J Farabaugh

Affiliations

Soon will be listed here.

Abstract

Programmed translational frameshift sites are sequences in mRNAs that promote frequent stochastic changes in translational reading frame allowing expression of alternative forms of protein products. The EST3 gene of Saccharomyces cerevisiae, encoding a subunit of telomerase, uses a programmed +1 frameshift site in its expression. We show that the site is complex, consisting of a heptameric sequence at which the frameshift occurs and a downstream 27-nucleotide stimulator sequence that increases frameshifting eightfold. The stimulator appears to be modular, composed of at least three separable domains. It increases frameshifting only when ribosomes pause at the frameshift site because of a limiting supply of a cognate aminoacyl-tRNA and not when pausing occurs at a nonsense codon. These data suggest that the EST3 stimulator may modulate access by aminoacyl-tRNAs to the ribosomal A site by interacting with several targets in a ribosome paused during elongation.

Citing Articles

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Ribosomes slide on lysine-encoding homopolymeric A stretches.

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