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Extracellular Polysaccharide is Required for Wild-type Virulence of Pseudomonas Solanacearum

Overview
Journal J Bacteriol
Specialty Microbiology
Date 1992 Feb 1
PMID 1732200
Citations 18
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Abstract

Several Pseudomonas solanacearum strains which produced no detectable extracellular polysaccharide (EPS) in planta had been reported to remain highly virulent when tested at high inoculum concentrations (P. Xu, M. Iwata, S. Leong, and L. Sequeira, J. Bacteriol. 172:3946-3951, 1990; P. Xu, S. Leong, and L. Sequeira, J. Bacteriol. 170:617-622, 1988). Two of these mutants, KD700 and KD710, have now been molecularly and genetically mapped to the EPSI gene cluster described by Denny and Baek (Mol. Plant-Microbe Interact. 4:198-206, 1991). When a range of inoculum concentrations was used, these two mutants and all other EPS-defective mutants tested were found to be reduced in virulence to eggplants and tobacco relative to the wild-type strain. Thus, EPS consistently is required for the wild-type level of virulence in P. solanacearum.

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References
1.
Kao C, Sequeira L . A gene cluster required for coordinated biosynthesis of lipopolysaccharide and extracellular polysaccharide also affects virulence of Pseudomonas solanacearum. J Bacteriol. 1991; 173(24):7841-7. PMC: 212575. DOI: 10.1128/jb.173.24.7841-7847.1991. View

2.
Cook D, Sequeira L . Genetic and biochemical characterization of a Pseudomonas solanacearum gene cluster required for extracellular polysaccharide production and for virulence. J Bacteriol. 1991; 173(5):1654-62. PMC: 207314. DOI: 10.1128/jb.173.5.1654-1662.1991. View

3.
Coplin D, Cook D . Molecular genetics of extracellular polysaccharide biosynthesis in vascular phytopathogenic bacteria. Mol Plant Microbe Interact. 1990; 3(5):271-9. DOI: 10.1094/mpmi-3-271. View

4.
Xu P, Iwata M, Leong S, Sequeira L . Highly virulent strains of Pseudomonas solanacearum that are defective in extracellular-polysaccharide production. J Bacteriol. 1990; 172(7):3946-51. PMC: 213378. DOI: 10.1128/jb.172.7.3946-3951.1990. View

5.
Staskawicz B, Dahlbeck D, Keen N, Napoli C . Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycinea. J Bacteriol. 1987; 169(12):5789-94. PMC: 214142. DOI: 10.1128/jb.169.12.5789-5794.1987. View