Interactions of Kid-Kis Toxin-antitoxin Complexes with the ParD Operator-promoter Region of Plasmid R1 Are Piloted by the Kis Antitoxin and Tuned by the Stoichiometry of Kid-Kis Oligomers

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2007 Feb 24

PMID 17317682

Citations 25

Authors

Maria C Monti

Ana M Hernandez-Arriaga

Monique B Kamphuis

Juan Lopez-Villarejo

Albert J R Heck

Rolf Boelens

Ramon Diaz-Orejas

Robert H H van den Heuvel

Affiliations

Soon will be listed here.

Abstract

The parD operon of Escherichia coli plasmid R1 encodes a toxin-antitoxin system, which is involved in plasmid stabilization. The toxin Kid inhibits cell growth by RNA degradation and its action is neutralized by the formation of a tight complex with the antitoxin Kis. A fascinating but poorly understood aspect of the kid-kis system is its autoregulation at the transcriptional level. Using macromolecular (tandem) mass spectrometry and DNA binding assays, we here demonstrate that Kis pilots the interaction of the Kid-Kis complex in the parD regulatory region and that two discrete Kis-binding regions are present on parD. The data clearly show that only when the Kis concentration equals or exceeds the Kid concentration a strong cooperative effect exists between strong DNA binding and Kid2-Kis2-Kid2-Kis2 complex formation. We propose a model in which transcriptional repression of the parD operon is tuned by the relative molar ratio of the antitoxin and toxin proteins in solution. When the concentration of the toxin exceeds that of the antitoxin tight Kid2-Kis2-Kid2 complexes are formed, which only neutralize the lethal activity of Kid. Upon increasing the Kis concentration, (Kid2-Kis2)n complexes repress the kid-kis operon.

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