An Antibody Derivative Expressed from Viral Vectors Passively Immunizes Pigs Against Transmissible Gastroenteritis Virus Infection when Supplied Orally in Crude Plant Extracts

Overview

Journal Plant Biotechnol J

Specialties Biology
Biotechnology

Date 2007 Feb 21

PMID 17309733

Citations 11

Authors

Wendy Monger

Josefa M Alamillo

Isabel Sola

Yolande Perrin

Marco Bestagno

Oscar R Burrone

Patricia Sabella

Joan Plana-Duran

Luis Enjuanes

Juan A Garcia

George P Lomonossoff

Affiliations

Soon will be listed here.

Abstract

To investigate the potential of antibody derivatives to provide passive protection against enteric infections when supplied orally in crude plant extracts, we have expressed a small immune protein (SIP) in plants using two different plant virus vectors based on potato virus X (PVX) and cowpea mosaic virus (CPMV). The epsilonSIP molecule consisted of a single-chain antibody (scFv) specific for the porcine coronavirus transmissible gastroenteritis virus (TGEV) linked to the epsilon-CH4 domain from human immunoglobulin E (IgE). In some constructs, the sequence encoding the epsilonSIP molecule was flanked by the leader peptide from the original murine antibody at its N-terminus and an endoplasmic reticulum retention signal (HDEL) at its C-terminus to allow the expressed protein to be directed to, and retained within, the endoplasmic reticulum. Western blot analysis of samples from Nicotiana clevelandii or cowpea tissue infected with constructs revealed the presence of SIP molecules which retained their ability to dimerize. The analysis of crude plant extracts revealed that the plant-expressed epsilonSIP molecules could bind to and neutralize TGEV in tissue culture, the levels of binding and neutralization reflecting the level of expression. Oral administration of crude extracts from SIP-expressing plant tissue to 2-day-old piglets demonstrated that the extracts which showed the highest levels of in vitro neutralization could also provide in vivo protection against challenge with TGEV.

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References

Castilla J, Sola I, Enjuanes L . Interference of coronavirus infection by expression of immunoglobulin G (IgG) or IgA virus-neutralizing antibodies. J Virol. 1997; 71(7):5251-8. PMC: 191761. DOI: 10.1128/JVI.71.7.5251-5258.1997. View

Batista F, Efremov D, Burrone O . Characterization of a second secreted IgE isoform and identification of an asymmetric pathway of IgE assembly. Proc Natl Acad Sci U S A. 1996; 93(8):3399-404. PMC: 39620. DOI: 10.1073/pnas.93.8.3399. View

Sola I, Castilla J, Pintado B, Whitelaw C, Clark A, Enjuanes L . Transgenic mice secreting coronavirus neutralizing antibodies into the milk. J Virol. 1998; 72(5):3762-72. PMC: 109598. DOI: 10.1128/JVI.72.5.3762-3772.1998. View

Stoger E, Ma J, Fischer R, Christou P . Sowing the seeds of success: pharmaceutical proteins from plants. Curr Opin Biotechnol. 2005; 16(2):167-73. DOI: 10.1016/j.copbio.2005.01.005. View

Li E, Pedraza A, Bestagno M, Mancardi S, Sanchez R, Burrone O . Mammalian cell expression of dimeric small immune proteins (SIP). Protein Eng. 1997; 10(6):731-6. DOI: 10.1093/protein/10.6.731. View

Castilla J, Pintado B, Sola I, Enjuanes L . Engineering passive immunity in transgenic mice secreting virus-neutralizing antibodies in milk. Nat Biotechnol. 1998; 16(4):349-54. PMC: 7097410. DOI: 10.1038/nbt0498-349. View

Stoger E, Sack M, Fischer R, Christou P . Plantibodies: applications, advantages and bottlenecks. Curr Opin Biotechnol. 2002; 13(2):161-6. DOI: 10.1016/s0958-1669(02)00303-8. View

Verch T, Yusibov V, KOPROWSKI H . Expression and assembly of a full-length monoclonal antibody in plants using a plant virus vector. J Immunol Methods. 1998; 220(1-2):69-75. DOI: 10.1016/s0022-1759(98)00149-5. View

Jimenez G, Correa I, Melgosa M, Bullido M, Enjuanes L . Critical epitopes in transmissible gastroenteritis virus neutralization. J Virol. 1986; 60(1):131-9. PMC: 253910. DOI: 10.1128/JVI.60.1.131-139.1986. View

10.

Ma J, Hikmat B, Wycoff K, Vine N, Chargelegue D, Yu L . Characterization of a recombinant plant monoclonal secretory antibody and preventive immunotherapy in humans. Nat Med. 1998; 4(5):601-6. DOI: 10.1038/nm0598-601. View

11.

Jones L, Hamilton A, Voinnet O, Thomas C, Maule A, Baulcombe D . RNA-DNA interactions and DNA methylation in post-transcriptional gene silencing. Plant Cell. 1999; 11(12):2291-301. PMC: 144133. DOI: 10.1105/tpc.11.12.2291. View

12.

Sune C, Jimenez G, Correa I, Bullido M, Gebauer F, Smerdou C . Mechanisms of transmissible gastroenteritis coronavirus neutralization. Virology. 1990; 177(2):559-69. PMC: 7131644. DOI: 10.1016/0042-6822(90)90521-r. View

13.

Correa I, Jimenez G, Sune C, Bullido M, Enjuanes L . Antigenic structure of the E2 glycoprotein from transmissible gastroenteritis coronavirus. Virus Res. 1988; 10(1):77-93. PMC: 7134057. DOI: 10.1016/0168-1702(88)90059-7. View

14.

Mechtcheriakova I, Eldarov M, Nicholson L, Shanks M, Skryabin K, Lomonossoff G . The use of viral vectors to produce hepatitis B virus core particles in plants. J Virol Methods. 2005; 131(1):10-5. DOI: 10.1016/j.jviromet.2005.06.020. View

15.

Zeitlin L, Olmsted S, Moench T, Co M, Martinell B, Paradkar V . A humanized monoclonal antibody produced in transgenic plants for immunoprotection of the vagina against genital herpes. Nat Biotechnol. 1998; 16(13):1361-4. DOI: 10.1038/4344. View

16.

McCormick A, Kumagai M, Hanley K, Turpen T, Hakim I, Grill L . Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants. Proc Natl Acad Sci U S A. 1999; 96(2):703-8. PMC: 15200. DOI: 10.1073/pnas.96.2.703. View

17.

Gebauer F, Posthumus W, Correa I, Sune C, Smerdou C, Sanchez C . Residues involved in the antigenic sites of transmissible gastroenteritis coronavirus S glycoprotein. Virology. 1991; 183(1):225-38. PMC: 7130809. DOI: 10.1016/0042-6822(91)90135-x. View

18.

Liu L, Lomonossoff G . Agroinfection as a rapid method for propagating Cowpea mosaic virus-based constructs. J Virol Methods. 2002; 105(2):343-8. DOI: 10.1016/s0166-0934(02)00121-0. View

19.

Liu L, Canizares M, Monger W, Perrin Y, Tsakiris E, Porta C . Cowpea mosaic virus-based systems for the production of antigens and antibodies in plants. Vaccine. 2005; 23(15):1788-92. DOI: 10.1016/j.vaccine.2004.11.006. View

20.

Borsi L, Balza E, Bestagno M, Castellani P, Carnemolla B, Biro A . Selective targeting of tumoral vasculature: comparison of different formats of an antibody (L19) to the ED-B domain of fibronectin. Int J Cancer. 2002; 102(1):75-85. DOI: 10.1002/ijc.10662. View