» Articles » PMID: 17307137

PGC-1alpha Over-expression Promotes Recovery from Mitochondrial Dysfunction and Cell Injury

Overview
Publisher Elsevier
Specialty Biochemistry
Date 2007 Feb 20
PMID 17307137
Citations 72
Authors
Affiliations
Soon will be listed here.
Abstract

Cell death from mitochondrial dysfunction and compromised bioenergetics is common after ischemia-reperfusion injury and toxicant exposure. Thus, promoting mitochondrial biogenesis is therapeutically attractive for sustaining oxidative phosphorylation and maintaining ATP-dependent cellular functions. Here, we evaluated increased mitochondrial biogenesis prior to or after oxidant exposure in primary cultures of renal proximal tubular cells (RPTC). Over-expression of the mitochondrial biogenesis regulator PPAR-gamma cofactor-1 alpha (PGC-1alpha) in control RTPC increased basal and uncoupled cellular respiration, ATP, and mitochondria. Increasing mitochondrial number/function prior to oxidant exposure did not preserve mitochondrial function, but potentiated dysfunction and cell death. However, increased mitochondrial biogenesis after oxidant injury accelerated recovery of mitochondrial function. In oxidant treated RPTC, mitochondrial protein expression was reduced by 50%. Also, ATP and cellular respiration decreased 48 h after oxidant exposure, whereas mitochondrial function in injured RPTC over-expressing PGC-1alpha returned to control values. Thus, up-regulation of mitochondrial biogenesis after oxidant exposure accelerates recovery of mitochondrial and cellular functions.

Citing Articles

The clinical efficacy of cGMP-specific sildenafil on mitochondrial biogenesis induction and renal damage in cats with acute on chronic kidney disease.

Maden M, Ider M, Or M, Dokuzeylul B, Gulersoy E, Kilickaya M BMC Vet Res. 2024; 20(1):499.

PMID: 39478527 PMC: 11526613. DOI: 10.1186/s12917-024-04345-9.


Peroxisome proliferator-activated receptor gamma coactivator-1 (PGC-1) family in physiological and pathophysiological process and diseases.

Qian L, Zhu Y, Deng C, Liang Z, Chen J, Chen Y Signal Transduct Target Ther. 2024; 9(1):50.

PMID: 38424050 PMC: 10904817. DOI: 10.1038/s41392-024-01756-w.


Electroacupuncture improves cognitive function in a rat model of mild traumatic brain injury by regulating the SIRT-1/PGC-1α/mitochondrial pathway.

Jin B, Gao Y, Fu Y, Zhang S, Zhang K, Su Y Chin Med J (Engl). 2024; 137(6):711-719.

PMID: 38384159 PMC: 10950173. DOI: 10.1097/CM9.0000000000003032.


The Protective Pathways Activated in Kidneys of αMUPA Transgenic Mice Following Ischemia\Reperfusion-Induced Acute Kidney Injury.

Alkhaleq H, Karram T, Fokra A, Hamoud S, Kabala A, Abassi Z Cells. 2023; 12(20).

PMID: 37887341 PMC: 10605904. DOI: 10.3390/cells12202497.


Mitochondrial quality control and its role in osteoporosis.

Yan C, Shi Y, Yuan L, Lv D, Sun B, Wang J Front Endocrinol (Lausanne). 2023; 14:1077058.

PMID: 36793284 PMC: 9922754. DOI: 10.3389/fendo.2023.1077058.