Distinct Roles of Broad Isoforms in Regulation of the 20-hydroxyecdysone Effector Gene, Vitellogenin, in the Mosquito Aedes Aegypti

Overview

Journal Mol Cell Endocrinol

Specialties Cell Biology
Endocrinology
Molecular Biology

Date 2007 Feb 17

PMID 17303321

Citations 22

Authors

Jinsong Zhu

Li Chen

Alexander S Raikhel

Affiliations

Soon will be listed here.

Abstract

We investigated the role of the mosquito broad (br) gene in regulating the 20-hydroxyecdysone (20E) effector Vitellogenin (Vg) gene. Injection of double-stranded RNA corresponding to the BR isoform Z2 led to a significant decrease in expression of the Vg gene at 8 and 24h post-blood meal. Knockdown of Z1 or Z4 resulted in enhanced Vg expression beyond its normal expression time. In vitro studies suggested that the effects of BR require its direct binding to the Vg promoter, as well as protein-protein interaction between BR and the ecdysone receptor complex. The BR isoforms are therefore essential for a proper stage-specific biological response to 20E in the adult female mosquito. In particular, the isoform Z2 is required for 20E-mediated activation of Vg, while isoforms Z1 and Z4 serve as repressors to ensure appropriate termination of Vg expression.

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References

Pierceall W, Li C, Biran A, Miura K, Raikhel A, Segraves W . E75 expression in mosquito ovary and fat body suggests reiterative use of ecdysone-regulated hierarchies in development and reproduction. Mol Cell Endocrinol. 1999; 150(1-2):73-89. DOI: 10.1016/s0303-7207(99)00022-2. View

Wang S, Li C, Zhu J, Miura K, Miksicek R, Raikhel A . Differential expression and regulation by 20-hydroxyecdysone of mosquito ultraspiracle isoforms. Dev Biol. 2000; 218(1):99-113. DOI: 10.1006/dbio.1999.9575. View

Riddiford L, Cherbas P, Truman J . Ecdysone receptors and their biological actions. Vitam Horm. 2000; 60:1-73. DOI: 10.1016/s0083-6729(00)60016-x. View

Mugat B, Brodu V, Kejzlarova-Lepesant J, Antoniewski C, Bayer C, Fristrom J . Dynamic expression of broad-complex isoforms mediates temporal control of an ecdysteroid target gene at the onset of Drosophila metamorphosis. Dev Biol. 2000; 227(1):104-17. DOI: 10.1006/dbio.2000.9879. View

Kokoza V, Martin D, Mienaltowski M, Ahmed A, Morton C, Raikhel A . Transcriptional regulation of the mosquito vitellogenin gene via a blood meal-triggered cascade. Gene. 2001; 274(1-2):47-65. DOI: 10.1016/s0378-1119(01)00602-3. View

Thummel C . Molecular mechanisms of developmental timing in C. elegans and Drosophila. Dev Cell. 2001; 1(4):453-65. DOI: 10.1016/s1534-5807(01)00060-0. View

Ward J, McConnell M, Carlile G, Pandolfi P, Licht J, Freedman L . The acute promyelocytic leukemia-associated protein, promyelocytic leukemia zinc finger, regulates 1,25-dihydroxyvitamin D(3)-induced monocytic differentiation of U937 cells through a physical interaction with vitamin D(3) receptor. Blood. 2001; 98(12):3290-300. DOI: 10.1182/blood.v98.12.3290. View

Zhou X, Riddiford L . Broad specifies pupal development and mediates the 'status quo' action of juvenile hormone on the pupal-adult transformation in Drosophila and Manduca. Development. 2002; 129(9):2259-69. DOI: 10.1242/dev.129.9.2259. View

Sun G, Zhu J, Li C, Tu Z, Raikhel A . Two isoforms of the early E74 gene, an Ets transcription factor homologue, are implicated in the ecdysteroid hierarchy governing vitellogenesis of the mosquito, Aedes aegypti. Mol Cell Endocrinol. 2002; 190(1-2):147-57. DOI: 10.1016/s0303-7207(01)00726-2. View

10.

Wang S, Li C, Sun G, Zhu J, Raikhel A . Differential expression and regulation by 20-hydroxyecdysone of mosquito ecdysteroid receptor isoforms A and B. Mol Cell Endocrinol. 2002; 196(1-2):29-42. DOI: 10.1016/s0303-7207(02)00225-3. View

11.

Zhu J, Miura K, Chen L, Raikhel A . Cyclicity of mosquito vitellogenic ecdysteroid-mediated signaling is modulated by alternative dimerization of the RXR homologue Ultraspiracle. Proc Natl Acad Sci U S A. 2003; 100(2):544-9. PMC: 141032. DOI: 10.1073/pnas.0235695100. View

12.

Hu X, Cherbas L, Cherbas P . Transcription activation by the ecdysone receptor (EcR/USP): identification of activation functions. Mol Endocrinol. 2003; 17(4):716-31. DOI: 10.1210/me.2002-0287. View

13.

Lea A . STUDIES ON THE DIETARY AND ENDOCRINE REGULATION OF AUTOGENOUS REPRODUCTION IN AEDES TAENIORHYNCHUS (WIED.). J Med Entomol. 1964; 1:40-4. DOI: 10.1093/jmedent/1.1.40. View

14.

Martin P, Delmotte M, Formstecher P, Lefebvre P . PLZF is a negative regulator of retinoic acid receptor transcriptional activity. Nucl Recept. 2003; 1(1):6. PMC: 212040. DOI: 10.1186/1478-1336-1-6. View

15.

Zhu J, Chen L, Raikhel A . Posttranscriptional control of the competence factor betaFTZ-F1 by juvenile hormone in the mosquito Aedes aegypti. Proc Natl Acad Sci U S A. 2003; 100(23):13338-43. PMC: 263810. DOI: 10.1073/pnas.2234416100. View

16.

Riddiford L, Hiruma K, Zhou X, Nelson C . Insights into the molecular basis of the hormonal control of molting and metamorphosis from Manduca sexta and Drosophila melanogaster. Insect Biochem Mol Biol. 2003; 33(12):1327-38. DOI: 10.1016/j.ibmb.2003.06.001. View

17.

Terashima J, Bownes M . Translating available food into the number of eggs laid by Drosophila melanogaster. Genetics. 2004; 167(4):1711-9. PMC: 1470999. DOI: 10.1534/genetics.103.024323. View

18.

Chen L, Zhu J, Sun G, Raikhel A . The early gene Broad is involved in the ecdysteroid hierarchy governing vitellogenesis of the mosquito Aedes aegypti. J Mol Endocrinol. 2004; 33(3):743-61. DOI: 10.1677/jme.1.01531. View

19.

Sun G, Zhu J, Chen L, Raikhel A . Synergistic action of E74B and ecdysteroid receptor in activating a 20-hydroxyecdysone effector gene. Proc Natl Acad Sci U S A. 2005; 102(43):15506-11. PMC: 1266084. DOI: 10.1073/pnas.0503501102. View

20.

Palli S, Hormann R, Schlattner U, Lezzi M . Ecdysteroid receptors and their applications in agriculture and medicine. Vitam Horm. 2006; 73:59-100. DOI: 10.1016/S0083-6729(05)73003-X. View