Characterization of T Antigen in Cells Infected with a Temperature-sensitive Mutant of Simian Virus 40

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1975 Jun 1

PMID 167188

Citations 29

Authors

T Kuchino

N Yamaguchi

Affiliations

Soon will be listed here.

Abstract

T antigen induced in African green monkey kidney cells by a temperature-sensitive mutant of simian virus 40, defective in a function required for cell transformation, was characterized. The number of T antigen-positive cells estimated by an immunofluorescent techniques was almost equal at permissive (32.5 C) and restrictive (38.5 C) temperatures, but was slightly reduced when the infected cells were incubated at a higher temperature (40.5 C). However, a complement fixation test indicated that the amount of T antigen induced by the mutant is not significantly different from that induced by wild-type virus at 40.5 C. These results suggest that the T antigen-inducing ability of the mutant is not defective. Two distinct molecular species of T antigen were induced by the mutant at the permissive temperature, whereas only one form was observed at the restrictive temperature. The larger molecular form (14 to 15S) induced by the mutant at the permissive temperature was more heat labile than that induced by wild-type virus, suggesting that the mutated gene product is a component of the larger molecular form.

Citing Articles

Complex of simian virus 40 large tumor antigen and 48,000-dalton host tumor antigen.

Greenspan D, Carroll R Proc Natl Acad Sci U S A. 1981; 78(1):105-9.

PMID: 6941238 PMC: 318999. DOI: 10.1073/pnas.78.1.105.

Subclasses of simian virus 40 large T antigen: differential binding of two subclasses of T antigen from productively infected cells to viral and cellular DNA.

Fanning E, WESTPHAL K, Brauer D, Corlin D EMBO J. 1982; 1(9):1023-8.

PMID: 6329720 PMC: 553156. DOI: 10.1002/j.1460-2075.1982.tb01290.x.

Oligomerization of simian virus 40 large T antigen is not necessarily repressed by temperature-sensitive A gene lesions.

Montenarh M, Kohler M, Henning R J Virol. 1984; 49(3):658-64.

PMID: 6321754 PMC: 255521. DOI: 10.1128/JVI.49.3.658-664.1984.

Different forms of simian virus 40 large tumor antigen varying in their affinities for DNA.

Gidoni D, Scheller A, Barnet B, Hantzopoulos P, Oren M, Prives C J Virol. 1982; 42(2):456-66.

PMID: 6283167 PMC: 256872. DOI: 10.1128/JVI.42.2.456-466.1982.

Detection and characterization of multiple forms of simian virus 40 large T antigen.

Fanning E, Nowak B, Burger C J Virol. 1981; 37(1):92-102.

PMID: 6261004 PMC: 170985. DOI: 10.1128/JVI.37.1.92-102.1981.

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